MTHFD1 Antibody

Western Blot: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - (1µg/ml) staining of Jurkat (A), MCF7 (B) and (2ug/ml) MOLT4 (C) cel lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunocytochemistry/Immunofluorescence: Goat Polyclonal MTHFD1 Antibody [NBP1-06054] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MTHFD1 Antibody Summary

• Description: Novus Biologicals Goat MTHFD1 Antibody (NBP1-06054) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-MTHFD1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence RGDLNDCFIPCTPK corresponding to internal region according to NP_005947.2.
• Epitope: RGDLNDCFIPCTPK
• Marker: Cytoplasm Marker
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MTHFD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Detection limit 1:1000
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 3.75 ug/ml
  • Western Blot 1 - 3 ug/ml
• Application Notes: Approx. 100kDa band observed in lysates of cell lines Jurkat and MOLT4 and approx.110kDa in lysates of cell line MCF7 (calculated MW of 102kDa according to NP_005947.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MTHFD1 Antibody

• C1-THF synthase
• EC 6.3.4.3
• methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolatecyclohydrolase, 10-formyltetrahydrofolate synthetase
• methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1
• MTHFCC-1-tetrahydrofolate synthase, cytoplasmic
• MTHFDcytoplasmic C-1-tetrahydrofolate synthase

Background

This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MTHFD1 Antibody (NBP1-06054)(1)

Publication using NBP1-06054

• Dorszewska J, Florczak J, Rozycka A et al. Oxidative DNA damage and level of thiols as related to polymorphisms of MTHFR, MTR, MTHFD1 in Alzheimer's and Parkinson's diseases. Acta Neurobiol Exp (Wars) 2007-01-01 [PMID: 17691219]

Bioinformatics

• Gene Symbol: MTHFD1
• Entrez:
  • 4522(Human)
  • 108156(Mouse)
  • 64300(Rat)
• Uniprot:
  • NP_005947.2(Human)