MTHFD1 Antibody

Images

 
Western Blot: MTHFD1 Antibody [NBP1-06054] (1ug/ml) staining of Human Substantia Nigra lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MTHFD1 Antibody Summary

Immunogen
Peptide with sequence RGDLNDCFIPCTPK corresponding to internal region according to NP_005947.2.
Epitope
RGDLNDCFIPCTPK
Marker
Cytoplasm Marker
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MTHFD1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:1000
Application Notes
WB: Approx. 110 kDa band observed in human brain (substantia nigra) lysates (calculated MW of 102 kDa band according to NP_005947.2).
Publications
Read Publication using NBP1-06054.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MTHFD1 Antibody

  • C1-THF synthase
  • EC 6.3.4.3
  • methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolatecyclohydrolase, 10-formyltetrahydrofolate synthetase
  • methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1
  • MTHFCC-1-tetrahydrofolate synthase, cytoplasmic
  • MTHFDcytoplasmic C-1-tetrahydrofolate synthase

Background

This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MTHFD1 Antibody (NBP1-06054)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MTHFD1
Entrez
Uniprot