MSX1 Antibody

Product Discontinued

MSX1 Antibody Summary

• Immunogen: Peptide with sequence TSLPLGVKVEDS-C corresponding to N-Terminus according to NP_002439.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MSX1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:64000
• Application Notes: WB: Preliminary experiments gave an approx. 55-60 kDa band in human muscle and heart lysates at 1 ug/ml. Further, this band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in scientific literature for the band we observe given the predicted MW of approx. 31.4 kDa band according to human NP_002439.2. IHC-P: Human prostate shows staining of nucleoli in a few epithelial cells. Data obtained from a previous batch (different goat).
• Control:

  MSX1 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Porcine, Bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MSX1 Antibody

• Homeobox protein Hox-7
• homeobox protein MSX-1
• HOX7
• HOX7homeobox 7
• HYD1
• HYD1msh homeobox homolog 1 (Drosophila)
• msh (Drosophila) homeo box homolog 1 (formerly homeo box 7)
• msh homeo box 1
• msh homeobox 1
• Msh homeobox 1-like protein
• msh homeobox homolog 1
• MSX1
• OFC5
• STHAG1

Background

This gene encodes a member of the muscle segment homeobox gene family. The encoded protein functions as a transcriptional repressor during embryogenesis through interactions with components of the core transcription complex and other homeoproteins. It may also have roles in limb-pattern formation, craniofacial development, particularly odontogenesis, and tumor growth inhibition. Mutations in this gene, which was once known as homeobox 7, have been associated with nonsyndromic cleft lip with or without cleft palate 5, Witkop syndrome, Wolf-Hirschom syndrome, and autosomoal dominant hypodontia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MSX1 Antibody (NB300-808)(1)

Publication using NB300-808

• Blin-Wakkach C, Lezot F, Ghoul-Mazgar S et al. Endogenous Msx1 antisense transcript: in vivo and in vitro evidences, structure, and potential involvement in skeleton development in mammals. Proc Natl Acad Sci U S A 2001-06-19 [PMID: 11390985]

Bioinformatics

• Gene Symbol: MSX1
• Entrez:
  • 4487(Human)
  • 17701(Mouse)
• Uniprot:
  • NP_002439.(Human)