MSH6 Antibody (MSH6/3086)

Western Blot: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Western Blot Analysis of HCT116 cell lysate using MSH6 Antibody (MSH6/3086).

Immunocytochemistry/Immunofluorescence: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Immunofluorescence staining of PFA-fixed MCF-7 cells with MSH6 Antibody (MSH6/3086) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with RedDot (Red).

Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MSH6 Antibody (MSH6/3086).

Flow Cytometry: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Flow Cytometric Analysis of PFA-fixed MCF-7 cells using MSH6 Mouse Monoclonal Antibody (MSH6/3086) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry-Paraffin: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Formalin-fixed, paraffin-embedded human Small Intestine stained with MSH6 Antibody (MSH6/3086).

Protein Array: MSH6 Antibody (MSH6/3086) [NBP2-79712] - Analysis of Protein Array containing >19,000 full-length human proteins using MSH6 Antibody (MSH6/3086).

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, MA
• Clone: MSH6/3086
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MSH6 Antibody (MSH6/3086) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79846)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant fragment of human MSH6 protein (around aa 374-540) (exact sequence is proprietary) (Uniprot: P52701)
• Localization: Nucleus
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSH6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order Ab without BSA.
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 163 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for MSH6 Antibody (MSH6/3086)

• DNA mismatch repair protein Msh6
• G/T mismatch-binding protein
• GTBPHNPCC5
• GTMBP
• hMSH6
• HSAP
• mutS (E. coli) homolog 6
• mutS homolog 6 (E. coli)
• MutS-alpha 160 kDa subunit
• p160
• sperm-associated protein

Background

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MSH6 Antibody (NBP2-79712). (Showing 1 - 1 of 1 FAQ).

1. I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.
   • Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.

Bioinformatics

• Gene Symbol: MSH6
• Uniprot:
  • P52701(Human)