MSH6 Antibody (CL13844) - Azide and BSA Free

Staining of human breast cancer (ductal carcinoma) shows strong nuclear positivity in tumor cells.

Analysis in human testis and skeletal muscle tissues using NBP3-43857 antibody. Corresponding MSH6 RNA-seq data are presented for the same tissues.

Staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Staining of human colon shows strong nuclear positivity in glandular cells.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Staining of human lymph node shows strong nuclear positivity in germinal center cells.

Staining of human ovarian cancer (high grade serous carcinoma) shows strong nuclear positivity in tumor cells.

Analysis in human cell line RH-30.

Staining of human testis cancer (embryonal carcinoma) shows strong nuclear positivity in tumor cells.

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL13844
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

MSH6 Antibody (CL13844) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse MSH6 Antibody (CL13844) - Azide and BSA Free (NBP3-24582) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a synthetic peptide of P52701, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSH6
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for MSH6 Antibody (CL13844) - Azide and BSA Free

• DNA mismatch repair protein Msh6
• G/T mismatch-binding protein
• GTBPHNPCC5
• GTMBP
• hMSH6
• HSAP
• mutS (E. coli) homolog 6
• mutS homolog 6 (E. coli)
• MutS-alpha 160 kDa subunit
• p160
• sperm-associated protein

Background

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MSH6 Antibody (NBP3-43857). (Showing 1 - 1 of 1 FAQ).

1. I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.
   • Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.

Bioinformatics

• Gene Symbol: MSH6