MSH6 Antibody (3E1)

Western Blot: MSH6 Antibody (3E1) [NBP2-37537] - Western blot analysis using MSH6 mouse mAb against HEK293 (1), HCT116 (2), A549 (3), A431 (4), MCF-7 (5) and HepG2 (6) cell lysate.

Immunocytochemistry/Immunofluorescence: MSH6 Antibody (3E1) [NBP2-37537] - Immunofluorescence analysis of Hela cells using MSH6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) [NBP2-37537] - Analysis of colon cancer tissues using MSH6 mouse mAb with DAB staining.

Flow Cytometry: MSH6 Antibody (3E1) [NBP2-37537] - Flow cytometric analysis of MCF-7 cells using MSH6 mouse mAb (green) and negative control (red).

Western Blot: MSH6 Antibody (3E1) [NBP2-37537] - Western blot analysis using MSH6 mAb against human MSH6 (AA: 217-395) recombinant protein. (Expected MW is 45.5 kDa)

Immunohistochemistry-Paraffin: MSH6 Antibody (3E1) [NBP2-37537] - Analysis of rectum cancer tissues using MSH6 mouse mAb with DAB staining.

ELISA: MSH6 Antibody (3E1) [NBP2-37537] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, IHC
• Clone: 3E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MSH6 Antibody (3E1) Summary

• Description: Novus Biologicals Mouse MSH6 Antibody (3E1) (NBP2-37537) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human MSH6 expressed in E. Coli.;
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MSH6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 160 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for MSH6 Antibody (3E1)

• DNA mismatch repair protein Msh6
• G/T mismatch-binding protein
• GTBPHNPCC5
• GTMBP
• hMSH6
• HSAP
• mutS (E. coli) homolog 6
• mutS homolog 6 (E. coli)
• MutS-alpha 160 kDa subunit
• p160
• sperm-associated protein

Background

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MSH6 Antibody (NBP2-37537). (Showing 1 - 1 of 1 FAQ).

1. I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.
   • Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.

Bioinformatics

• Gene Symbol: MSH6