MSH2 Antibody (2L9I4)

Western Blot: MSH2 Antibody (2L9I4) [NBP3-16692] - Western blot analysis of extracts of various cell lines, using MSH2 Rabbit mAb (NBP3-16692) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry-Paraffin: MSH2 Antibody (2L9I4) [NBP3-16692] - Immunohistochemistry of paraffin-embedded human colon using MSH2 Rabbit mAb (NBP3-16692) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: MSH2 Antibody (2L9I4) [NBP3-16692] - Immunohistochemistry of paraffin-embedded rat ovary using MSH2 Rabbit mAb (NBP3-16692) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 2L9I4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MSH2 Antibody (2L9I4) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSH2 (P43246). MAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRV
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MSH2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MSH2 Antibody (2L9I4)

• COCA1
• DNA mismatch repair protein Msh2
• FCC1
• hMSH2
• HNPCC
• HNPCC1
• HNPCC1mutS (E. coli) homolog 2 (colon cancer, nonpolyposis type 1)
• LCFS2
• MSH2
• mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• MutS protein homolog 2

Background

MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSH2