MRPS18B Antibody - BSA Free

Western Blot: MRPS18B Antibody [NBP2-15024] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: MRPS18B Antibody [NBP2-15024] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.

• Reactivity: Hu, Po, Bv, Pm, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MRPS18B Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MRPS18B Antibody - BSA Free (NBP2-15024) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human MRPS18B. The exact sequence is proprietary.
• Predicted Species: Porcine (90%), Bovine (90%), Rhesus Macaque (94%), Primate (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPS18B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (98%), Mouse (85%), Rat (85%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for MRPS18B Antibody - BSA Free

• 28S ribosomal protein S18-2, mitochondrial
• C6orf1428S ribosomal protein S18b, mitochondrial
• HSPC183
• HumanS18a
• mitochondrial ribosomal protein S18-2
• mitochondrial ribosomal protein S18B
• MRP-S18-2
• MRPS18-2DKFZp564H0223
• MRP-S18-b
• mrps18-b
• PTD017
• S18amt
• S18mt-b

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MRPS18B