MRCK Antibody - BSA Free

Immunoprecipitation: MRCK Antibody [NBP1-46823] - Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MRCK antibody NBP1-46823 used for IP at 3 ug/mg lysate. MRCK was also immunoprecipitated by rabbit anti-MRCK antibody NBP1-46822, which recognizes an upstream epitope. For blotting immunoprecipitated MRC, NBP1-46822 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunoprecipitation: MRCK Antibody [NBP1-46823] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TORC3 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TORC3 was also immunoprecipitated by rabbit anti-TORC3 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

• Reactivity: Hu
• Applications: IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

MRCK Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MRCK Antibody - BSA Free (NBP1-46823) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 1610 and 1660 of human myotonic dystrophy protein kinase-like alpha using the numbering given in entry CAH71185.1 (GeneID 8476).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC42BPA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MRCK Antibody - BSA Free

• CDC42 binding protein kinase alpha (DMPK-like)
• CDC42 binidng protein kinase beta
• CDC42-binding protein kinase alpha (DMPK-like)
• CDC42-binding protein kinase alpha
• DMPK-like alpha
• EC 2.7.11
• EC 2.7.11.1
• KIAA0451DKFZp686L1738
• MRCK alpha
• MRCK
• MRCKADKFZp686P1738
• Myotonic dystrophy kinase-related CDC42-binding kinase alpha
• myotonic dystrophy kinase-related CDC42-binding protein kinase alpha
• Myotonic dystrophy protein kinase-like alpha
• PK428FLJ23347
• serine/threonine-protein kinase MRCK alpha
• ser-thr protein kinase PK428
• ser-thr protein kinase related to the myotonic dystrophy protein kinase

Background

The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC42BPA