Mouse Thrombopoietin Quantikine ELISA Kit, 2 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse Thrombopoietin Quantikine ELISA Kit, 2 Plate Summary

• Background: The Quantikine Mouse Tpo Immunoassay is a 4.5 hour solid phase ELISA designed to measure Tpo in cell culture supernates, serum, and plasma. It contains recombinant mouse Tpo and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse Tpo showed dose-response curves that were parall...el to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Tpo.
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• Specificity: Natural and recombinant mouse Tpo
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Thpo

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Thrombopoietin Quantikine ELISA Kit, 2 Plate

• Megakaryocyte colony-stimulating factor
• Megakaryocyte growth and development factor
• megakaryocyte stimulating factor
• MGDF
• MGDFC-mpl ligand
• MKCSF
• MK-CSF
• ML
• MPL ligand
• MPLLG
• MPLLGMGC163194
• Myeloproliferative leukemia virus oncogene ligand
• THCYT1
• THPO
• thrombopoietin nirs variant 1
• Thrombopoietin
• Tpo
• TPOMKCSF

Background

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders. The 353 amino acid (aa) human Tpo precursor is cleaved to yield the 332 aa mature protein. Mature human Tpo shares approximately 70% aa sequence homology with mouse and rat Tpo. It is an 80 - 85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites. Tissue specific alternate splicing of human Tpo generates multiple isoforms with internal deletions, insertions, and/or C-terminal substitutions. Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors. Several other cytokines can promote these functions as well but only in cooperation with Tpo. Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage. Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation. It is cleaved by platelet-derived thrombin following Arg191 within the C-terminal domain and subsequently at other sites upon extended digestion. Full length Tpo and shorter forms circulate in the plasma. The C-terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation. Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF induced signaling.

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-Mpl), is a type I transmembrane protein that is a member of the hematopoietin/cytokine receptor superfamily. As a consequence of alternative splicing, there are four identified mRNA variants. The functional receptor is encoded by the P isoform of mRNA. The human and mouse receptors share approximately 81% amino acid sequence identity. Tpo R is expressed at low levels in various cell types, including hematopoietic progenitor cells, megakaryocytes and platelets.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Thrombopoietin/THPO (MTP00)(45)

Publications using MTP00

• Karakas, D;Li, J;Ma, W;Wu, XG;Khoury, CJ;Masoud, M;Hoard, B;Liu, YJ;Zhu, G;Chen, J;Sim, M;Shen, C;López, JA;MacParland, SA;Kahr, WHA;Ni, H; Kupffer cells are essential for platelet-mediated thrombopoietin generation in the liver Proceedings of the National Academy of Sciences of the United States of America 2025-09-23 [PMID: 40961144] (Mouse)
• Everett, LA;Lin, Z;Friedman, A;Tang, VT;Myers, G;Balbin-Cuesta, G;King, R;Zhu, G;McGee, B;Khoriaty, R; LMAN1 serves as a cargo receptor for thrombopoietin JCI insight 2024-11-05 [PMID: 39499573] (Mouse)
• Gaertner, F;Ishikawa-Ankerhold, H;Stutte, S;Fu, W;Weitz, J;Dueck, A;Nelakuditi, B;Fumagalli, V;van den Heuvel, D;Belz, L;Sobirova, G;Zhang, Z;Titova, A;Navarro, AM;Pekayvaz, K;Lorenz, M;von Baumgarten, L;Kranich, J;Straub, T;Popper, B;Zheden, V;Kaufmann, WA;Guo, C;Piontek, G;von Stillfried, S;Boor, P;Colonna, M;Clau beta , S;Schulz, C;Brocker, T;Walzog, B;Scheiermann, C;Aird, WC;Nerlov, C;Stark, K;Petzold, T;Engelhardt, S;Sixt, M;Hauschild, R;Rudelius, M;Oostendorp, RAJ;Iannacone, M;Heinig, M;Massberg, S; Plasmacytoid dendritic cells control homeostasis of megakaryopoiesis Nature 2024-07-01 [PMID: 38987596] (Transgenic Mouse)
• Becker, IC;Wilkie, AR;Nikols, E;Carminita, E;Roweth, HG;Tilburg, J;Sciaudone, AR;Noetzli, LJ;Fatima, F;Couldwell, G;Ray, A;Mogilner, A;Machlus, KR;Italiano, JE; Cell cycle-dependent centrosome clustering precedes proplatelet formation Science advances 2024-06-21 [PMID: 38896608] (Mouse)
• Behrens, K;Kauppi, M;Viney, EM;Kueh, AJ;Hyland, CD;Willson, TA;Salleh, L;de Graaf, CA;Babon, JJ;Herold, MJ;Nicola, NA;Alexander, WS; Differential in vivo roles of Mpl cytoplasmic tyrosine residues in murine hematopoiesis and myeloproliferative disease Leukemia 2024-03-15 [PMID: 38491305] (Transgenic Mouse)
• Holmes-Hampton, GP;Kumar, VP;Biswas, S;Stone, S;Sharma, NK;Legesse, B;Vercellino, J;Guha, C;Eichenbaum, G;Ghosh, SP; PEGylated thrombopoietin mimetic, JNJ?26366821 a novel prophylactic radiation countermeasure for acute radiation injury Scientific reports 2023-09-14 [PMID: 37709916] (Mouse)
• Becker, HJ;Ishida, R;Wilkinson, AC;Kimura, T;Lee, MSJ;Coban, C;Ota, Y;Tanaka, Y;Roskamp, M;Sano, T;Tojo, A;Kent, DG;Yamazaki, S; Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion Cell stem cell 2023-07-06 [PMID: 37385251] (Mouse)
• Kocatürk, B;Lee, Y;Nosaka, N;Abe, M;Martinon, D;Lane, ME;Moreira, D;Chen, S;Fishbein, MC;Porritt, RA;Franklin, BS;Noval Rivas, M;Arditi, M; Platelets exacerbate cardiovascular inflammation in a murine model of Kawasaki disease vasculitis JCI insight 2023-06-06 [PMID: 37279077] (Mouse)
• Jeong, SH;Park, JY;Ryu, YB;Kim, WS;Lee, IC;Kim, JH;Kim, D;Ha, JH;Lee, BW;Nam, J;Cho, KO;Kwon, HJ; Myristica fragrans Extract Inhibits Platelet Desialylation and Activation to Ameliorate Sepsis-Associated Thrombocytopenia in a Murine CLP-Induced Sepsis Model International journal of molecular sciences 2023-05-16 [PMID: 37240208] (Mouse)
• SY Heazlewood, T Ahmad, B Cao, H Cao, M Domingues, X Sun, CK Heazlewood, S Li, B Williams, M Fulton, JF White, T Nebl, CM Nefzger, JM Polo, BT Kile, F Kraus, MT Ryan, YB Sun, PFM Choong, SL Ellis, ML Anko, SK Nilsson High ploidy large cytoplasmic megakaryocytes are hematopoietic stem cells regulators and essential for platelet production Nature Communications, 2023-04-13;14(1):2099. 2023-04-13 [PMID: 37055407] (Mouse)
• HG Roweth, MW Malloy, GJ Goreczny, IC Becker, Q Guo, EA Mittendorf, JE Italiano, SS McAllister, EM Battinelli Pro-inflammatory megakaryocyte gene expression in murine models of breast cancer Science Advances, 2022-10-12;8(41):eabo5224. 2022-10-12 [PMID: 36223471] (Mouse)
• MGE Rommel, L Walz, F Fotopoulou, S Kohlscheen, F Schenk, C Miskey, L Botezatu, Y Krebs, IM Voelker, K Wittwer, T Holland-Le, Z Ivics, V von Messli, MAG Essers, MD Milsom, CK Pfaller, U Modlich Influenza A virus infection instructs hematopoiesis to megakaryocyte-lineage output Cell Reports, 2022-10-04;41(1):111447. 2022-10-04 [PMID: 36198277] (Mouse)
• JL Spivak, A Merchant, DM Williams, O Rogers, W Zhao, A Duffield, LS Resar, AR Moliterno, ZJ Zhao Thrombopoietin is required for full phenotype expression in a JAK2V617F transgenic mouse model of polycythemia vera PLoS ONE, 2020-06-01;15(6):e0232801. 2020-06-01 [PMID: 32479500] (Transgenic Mouse)
• CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells Cell Rep, 2019-09-24;28(13):3329-3337.e5. 2019-09-24 [PMID: 31553904] (Mouse)
• B Hurtado, M Trakala, P Ximénez-Em, A El Bakkali, D Partida, B Sanz-Casti, M Álvarez-Fe, M Maroto, R Sánchez-Ma, L Martínez, J Muñoz, P García de, M Malumbres Thrombocytopenia-associated mutations in Ser/Thr kinase MASTL deregulate actin cytoskeleton dynamics in platelets J. Clin. Invest., 2018-10-29;0(0):. 2018-10-29 [PMID: 30252678] (Mouse)
• Q Ma, C Zhu, W Zhang, N Ta, R Zhang, L Liu, D Feng, H Cheng, J Liu, Q Chen Mitochondrial PIP3-binding protein FUNDC2 supports platelet survival via AKT signaling pathway Cell Death Differ., 2018-05-21;0(0):. 2018-05-21 [PMID: 29786068] (Mouse)
• S Ding, M Wang, S Fang, H Xu, H Fan, Y Tian, Y Zhai, S Lu, X Qi, F Wei, G Sun, X Sun D-dencichine Regulates Thrombopoiesis by Promoting Megakaryocyte Adhesion, Migration and Proplatelet Formation Front Pharmacol, 2018-04-03;9(0):297. 2018-04-03 [PMID: 29666579] (Mouse)
• MB Alvarez, L Xu, P Childress, KA Maupin, SFG Mohamad, B Chitteti, E Himes, DJ Olivos Iii, YH Cheng, SJ Conway, EF Srour, MA Kacena Megakaryocyte and Osteoblast Interactions Modulate Bone Mass and Hematopoiesis Stem Cells Dev., 2018-05-15;0(0):. 2018-05-15 [PMID: 29631496] (Mouse)
• YX Tan, H Cui, LM Wan, F Gong, X Zhang, I Vlodavsky, JP Li Overexpression of heparanase in mice promoted megakaryopoiesis Glycobiology, 2018-05-01;0(0):. 2018-05-01 [PMID: 29471321] (ELISA Capture, Mouse)
• M Zingariell, L Sancillo, F Martelli, F Ciaffoni, M Marra, L Varricchio, RA Rana, C Zhao, JD Crispino, AR Migliaccio The thrombopoietin/MPL axis is activated in the Gata1(low) mouse model of myelofibrosis and is associated with a defective RPS14 signature Blood Cancer J, 2017-06-16;7(6):e572. 2017-06-16 [PMID: 28622305] (Mouse)
• Repercussion of Megakaryocyte-Specific Gata1 Loss on Megakaryopoiesis and the Hematopoietic Precursor Compartment PLoS ONE, 2016-05-06;11(5):e0154342. 2016-05-06 [PMID: 27152938] (Mouse)
• Bender M, Giannini S, Grozovsky R, Jonsson T, Christensen H, Pluthero F, Ko A, Mullally A, Kahr W, Hoffmeister K, Falet H Dynamin 2-dependent endocytosis is required for normal megakaryocyte development in mice. Blood, 2014-12-02;125(6):1014-24. 2014-12-02 [PMID: 25468568] (Mouse)
• Nakamura-Ishizu , Ayako, Takubo , Keiyo, Fujioka , Masato, Suda , Toshio Megakaryocytes are essential for HSC quiescence through the production of thrombopoietin. Biochem Biophys Res Commun, 2014-10-24;454(2):353-7. 2014-10-24 [PMID: 25451253] (Mouse)
• Greene T, Lyde R, Bailey S, Lambert M, Zhai L, Sabatino D, Camire R, Arruda V, Poncz M Apoptotic effects of platelet factor VIII on megakaryopoiesis: implications for a modified human FVIII for platelet-based gene therapy. J Thromb Haemost, 2014-11-04;12(12):2102-12. 2014-11-04 [PMID: 25287191] (Mouse)
• Molina-Ortiz P, Polizzi S, Ramery E, Gayral S, Delierneux C, Oury C, Iwashita S, Schurmans S Rasa3 controls megakaryocyte Rap1 activation, integrin signaling and differentiation into proplatelet. PLoS Genet, 2014-06-26;10(6):e1004420. 2014-06-26 [PMID: 24967784] (Mouse)
• Josefsson , Emma C, Burnett , Deborah, Lebois , Marion, Debrincat , Marlyse, White , Michael, Henley , Katya J, Lane , Rachael, Moujalled , Diane, Preston , Simon P, O'Reilly , Lorraine, Pellegrini , Marc, Metcalf , Donald, Strasser , Andreas, Kile , Benjamin Platelet production proceeds independently of the intrinsic and extrinsic apoptosis pathways. Nat Commun, 2014-03-17;5(0):3455. 2014-03-17 [PMID: 24632563] (Mouse)
• Grover , Amit, Mancini , Elena, Moore , Susan, Mead , Adam J, Atkinson , Deborah, Rasmussen , Kasper D, O'Carroll , Donal, Jacobsen , Sten Eir, Nerlov , Claus Erythropoietin guides multipotent hematopoietic progenitor cells toward an erythroid fate. J Exp Med, 2014-02-03;211(2):181-8. 2014-02-03 [PMID: 24493804] (Mouse)
• Hong S, Dvorak-Ewell M, Stevens H, Barish G, Castro G, Nofsinger R, Frangos J, Shoback D, Evans R Rescue of a primary myelofibrosis model by retinoid-antagonist therapy. Proc Natl Acad Sci U S A, 2013-11-04;110(47):18820-5. 2013-11-04 [PMID: 24191050] (Mouse)
• Zhang L, Orban M, Lorenz M, Barocke V, Braun D, Urtz N, Schulz C, von Bruhl M, Tirniceriu A, Gaertner F, Proia R, Graf T, Bolz S, Montanez E, Prinz M, Muller A, von Baumgarten L, Billich A, Sixt M, Fassler R, von Andrian U, Junt T, Massberg S A novel role of sphingosine 1-phosphate receptor S1pr1 in mouse thrombopoiesis. J Exp Med, 2012-11-12;209(12):2165-81. 2012-11-12 [PMID: 23148237] (Mouse)
• Mcl-1 and Bcl-xL regulate Bak/Bax-dependent apoptosis of the megakaryocytic lineage at multistages. Cell Death Differ., 2012-07-13;19(11):1856-69. 2012-07-13 [PMID: 22790873] (Mouse)
• Mcl-1 and Bcl-x(L) coordinately regulate megakaryocyte survival. Blood, 2012-02-28;119(24):5850-8. 2012-02-28 [PMID: 22374700] (Mouse)
• Salojin KV, Cabrera RM, Sun W A mouse model of hereditary folate malabsorption: deletion of the PCFT gene leads to systemic folate deficiency. Blood, 2011-02-23;117(18):4895-904. 2011-02-23 [PMID: 21346251] (Mouse)
• Heckl D, Wicke DC, Brugman MH, Meyer J, Schambach A, Busche G, Ballmaier M, Baum C, Modlich U Lentiviral gene transfer regenerates hematopoietic stem cells in a mouse model for Mpl-deficient aplastic anemia. Blood, 2011-02-02;117(14):3737-47. 2011-02-02 [PMID: 21289307] (Mouse)
• Peeters K, Loyen S, Van Kerckhoven S, Stoffels K, Hoylaerts MF, Van Geet C, Freson K Thrombopoietic effect of VPAC1 inhibition during megakaryopoiesis. Br. J. Haematol., 2010-08-05;151(1):54-61. 2010-08-05 [PMID: 20735399] (Mouse)
• Kruse EA, Loughran SJ, Baldwin TM, Josefsson EC, Ellis S, Watson DK, Nurden P, Metcalf D, Hilton DJ, Alexander WS, Kile BT Dual requirement for the ETS transcription factors Fli-1 and Erg in hematopoietic stem cells and the megakaryocyte lineage. Proc. Natl. Acad. Sci. U.S.A., 2009-07-31;106(33):13814-9. 2009-07-31 [PMID: 19666492] (Mouse)
• Eckly A, Strassel C, Freund M, Cazenave JP, Lanza F, Gachet C, Leon C Abnormal megakaryocyte morphology and proplatelet formation in mice with megakaryocyte-restricted MYH9 inactivation. Blood, 2008-11-04;113(14):3182-9. 2008-11-04 [PMID: 18984861] (Mouse)
• Lannutti BJ, Epp A, Roy J, Chen J, Josephson NC Incomplete restoration of Mpl expression in the mpl-/- mouse produces partial correction of the stem cell-repopulating defect and paradoxical thrombocytosis. Blood, 2008-09-16;113(8):1778-85. 2008-09-16 [PMID: 18796624] (Mouse)
• Zhang P, Nelson S, Bagby GJ, Siggins R, Shellito JE, Welsh DA The lineage-c-Kit+Sca-1+ cell response to Escherichia coli bacteremia in Balb/c mice. Stem Cells, 2008-05-15;26(7):1778-86. 2008-05-15 [PMID: 18483422] (Mouse)
• Xing S, Wanting TH, Zhao W, Ma J, Wang S, Xu X, Li Q, Fu X, Xu M, Zhao ZJ Transgenic expression of JAK2V617F causes myeloproliferative disorders in mice. Blood, 2008-03-11;111(10):5109-17. 2008-03-11 [PMID: 18334677] (Mouse)
• Tiedt R, Hao-Shen H, Sobas MA, Looser R, Dirnhofer S, Schwaller J, Skoda RC Ratio of mutant JAK2-V617F to wild-type Jak2 determines the MPD phenotypes in transgenic mice. Blood, 2007-12-26;111(8):3931-40. 2007-12-26 [PMID: 18160670] (Mouse)
• Kile BT, Panopoulos AD, Stirzaker RA, Hacking DF, Tahtamouni LH, Willson TA, Mielke LA, Henley KJ, Zhang JG, Wicks IP, Stevenson WS, Nurden P, Watowich SS, Justice MJ Mutations in the cofilin partner Aip1/Wdr1 cause autoinflammatory disease and macrothrombocytopenia. Blood, 2007-05-21;110(7):2371-80. 2007-05-21 [PMID: 17515402] (Mouse)
• Wu Y, Welte T, Michaud M, Madri JA PECAM-1: a multifaceted regulator of megakaryocytopoiesis. Blood, 2007-04-05;110(3):851-9. 2007-04-05 [PMID: 17412889] (Mouse)
• Kaluzhny Y, >”eêCY¦>”e, Yu G, Sun S, Toselli PA, Nieswandt B, Jackson CW, Ravid K BclxL overexpression in megakaryocytes leads to impaired platelet fragmentation. 2002-09-01;100(5):1670-8. 2002-09-01 [PMID: 12176887] (Mouse)
• Kaser A, Brandacher G, Steurer W, Kaser S, Offner FA, Zoller H, Theurl I, Widder W, Molnar C, Ludwiczek O, Atkins MB, Mier JW, Tilg H Interleukin-6 stimulates thrombopoiesis through thrombopoietin: role in inflammatory thrombocytosis. Blood, 2001-11-01;98(9):2720-5. 2001-11-01 [PMID: 11675343] (Mouse)
• Shih CC, Hu MC, Hu J A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells. Blood, 2000-03-15;95(6):1957-66. 2000-03-15 [PMID: 10706861] (Mouse)

FAQs for Thrombopoietin/THPO (MTP00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Thpo