Mouse/Rat IGF-I/IGF-1 DuoSet ELISA, 15 Plate

• Reactivity: Mu, Rt
• Applications: ELISA
• Conjugate: Biotin

Mouse/Rat IGF-I/IGF-1 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Igf1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat IGF-I/IGF-1 DuoSet ELISA, 15 Plate

• IBP1
• IGF1
• IGF-1
• IGF1A
• IGFI
• IGF-I
• IGF-IA
• IGF-IB
• insulin-like growth factor 1 (somatomedin C)
• insulin-like growth factor 1
• insulin-like growth factor I
• insulin-like growth factor IA
• insulin-like growth factor IB
• Mechano growth factor
• MGF
• Somatomedin A
• Somatomedin C
• somatomedin-C

Background

Insulin-like Growth Factor 1 (IGF-1), also known as somatomedin C, is a member of the insulin superfamily. It was originally discovered as a mediator of growth hormone actions on somatic cell growth, but has also been shown to be an important regulator of cell metabolism, differentiation and survival. IGF-1 is synthesized as a preproprotein that is proteolytically cleaved to generate the mature protein linked by three disulfide bonds. Mature IGF-1 is highly conserved among large mammals, with 100% sequence identity between the human, bovine, porcine, equine, and canine proteins. 

Mature mouse IGF-1 is a non-glycosylated, 70 amino acid (aa) secreted polypeptide that is derived from either a 153 aa or a 159 aa preproprotein. It shares 99% and 94% aa sequence identity with rat and human IGF-1, respectively. IGF-1 is synthesized in the liver and other tissues. It is found in blood and other body fluids as a complex with specific high affinity IGF binding proteins (IGFBP-1 to -6). The IGFBPs are expressed in specific patterns during development. They are modulators of IGF actions, which control IGF bioavailability to specific cell-surface receptors. Their functions are further regulated by IGFBP proteases, which proteolytically cleave the IGFBPs to lower the affinity with which they bind IGFs and increase IGF bioavailability. Some IGFBPs also have IGF-Independent effects on cell functions. IGF-Independent circulates primarily as a ternary complex with IGFBP-3 or IGFBP-5 and the acid-labile subunit (ALS). Some IGF-1 is also present in binary complexes with other IGFBPs. Whereas the ternary complexes are generally restricted to the vasculature, the binary complexes freely enter the tissues. 

IGF-1 actions are mediated by two ubiquitously expressed receptor tyrosine kinases: IGF-I R and Insulin R/CD220. IGF-I R and Insulin R are disulfide-linked heterotetrameric complexes that consist of two alpha and two beta subunits. For both of these receptors, the prepro proteins are cleaved to produce extracellular alpha subunits which contain a cysteine-rich region and ligand-binding fibronectin type III (FN-III) domains, and beta subunits which contain an extracellular FN-III domain, transmembrane, and cytoplasmic tyrosine kinase domains. A hybrid complex containing one IGF-I R and one Insulin R also serves as a functional high affinity receptor for IGF-1. IGF-I R-Insulin R hybrids respond primarily to IGF-1, potentially downregulating the cellular response to Insulin. IGF signaling is also modulated by IGF binding proteins and the scavenger receptor, IGF-I I R.

Publications for IGF-I/IGF-1 (DY791)(18)

Publications using DY791

• AS Naik, SQ Wang, M Chowdhury, J Aqeel, CL O'Connor, JE Wiggins, M Bitzer, RC Wiggins Critical timing of ACEi initiation prevents compensatory glomerular hypertrophy in the remaining single kidney Scientific Reports, 2021;11(1):19605. 2021 [PMID: 34599260] (Rat)
• MD Rogowska, UNV Pena, N Binning, JK Christians Recovery of the maternal skeleton after lactation is impaired by advanced maternal age but not by reduced IGF availability in the mouse PLoS ONE, 2021;16(9):e0256906. 2021 [PMID: 34469481] (Mouse)
• S Keshvari, M Caruso, N Teakle, L Batoon, A Sehgal, OL Patkar, M Ferrari-Ce, CE Snell, C Chen, A Stevenson, FM Davis, SJ Bush, C Pridans, KM Summers, AR Pettit, KM Irvine, DA Hume CSF1R-dependent macrophages control postnatal somatic growth and organ maturation PloS Genetics, 2021;17(6):e1009605. 2021 [PMID: 34081701] (Rat)
• M Hong, IH Han, I Choi, N Cha, W Kim, SK Kim, H Bae Magnoliae Cortex Alleviates Muscle Wasting by Modulating M2 Macrophages in a Cisplatin-Induced Sarcopenia Mouse Model International Journal of Molecular Sciences, 2021;22(6):. 2021 [PMID: 33804803] (Mouse)
• J Xu, X Wang, J Chen, S Chen, Z Li, H Liu, Y Bai, F Zhi Embryonic stem cell-derived mesenchymal stem cells promote colon epithelial integrity and regeneration by elevating circulating IGF-1 in colitis mice Theranostics, 2020;10(26):12204-12222. 2020 [PMID: 33204338] (Mouse)
• X Niu, Y Zhao, N Yang, X Zhao, W Zhang, X Bai, A Li, W Yang, L Lu Proteasome Activation by Insulin-like Growth Factor-1/Nuclear Factor Erythroid 2-related Factor 2 Signaling Promotes Exercise-induced Neurogenesis Stem Cells, 2019;0(0):. 2019 [PMID: 31648402] (Mouse)
• F Ascenzi, L Barberi, G Dobrowolny, A Villa Nova, C Nicoletti, E Rizzuto, N Rosenthal, BM Scicchitan, A Musarò Effects of IGF-1 isoforms on muscle growth and sarcopenia Aging Cell, 2019;0(0):e12954. 2019 [PMID: 30953403] (Mouse)
• KME Andersson, C Wasén, L Juzokaite, L Leifsdotti, MC Erlandsson, ST Silfverswä, A Stokowska, M Pekna, M Pekny, K Olmarker, RA Heckemann, M Kalm, MI Bokarewa Inflammation in the hippocampus affects IGF1 receptor signaling and contributes to neurological sequelae in rheumatoid arthritis Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 30509997] (Mouse)
• V Giambra, S Gusscott, D Gracias, R Song, SH Lam, P Panelli, K Tyshchenko, CE Jenkins, C Hoofd, A Lorzadeh, A Carles, M Hirst, CJ Eaves, AP Weng Epigenetic Restoration of Fetal-like IGF1 Signaling Inhibits Leukemia Stem Cell Activity Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30269902] (Mouse)
• L R Batista-Si Mycobacterium leprae-induced Insulin-like Growth Factor I attenuates antimicrobial mechanisms, promoting bacterial survival in macrophages Sci Rep, 2016;6(0):27632. 2016 [PMID: 27282338] (Mouse)
• Liu , Xianglan, McMurphy , Travis, Xiao , Run, Slater , Andrew, Huang , Wei, Cao , Lei Hypothalamic gene transfer of BDNF inhibits breast cancer progression and metastasis in middle age obese mice. Mol Ther, 2014;22(7):1275-84. 2014 [PMID: 24637454] (Mouse)
• Hansen , Michelle, Chen , Hui, Jones , Jessica, Langenbach , Shenna Y, Vlahos , Ross, Gualano , Rosa C, Morris , Margaret, Anderson , Gary P The lung inflammation and skeletal muscle wasting induced by subchronic cigarette smoke exposure are not altered by a high-fat diet in mice. PLoS ONE, 2013;8(11):e80471. 2013 [PMID: 24260397] (Mouse)
• Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation, 2012;9(0):65. 2012 [PMID: 22483094] (Mouse)
• Kelly-Spratt KS, Pitteri SJ, Gurley KE, Liggitt D, Chin A, Kennedy J, Wong CH, Zhang Q, Buson TB, Wang H, Hanash SM, Kemp CJ Plasma proteome profiles associated with inflammation, angiogenesis, and cancer. PLoS ONE, 2011;6(5):e19721. 2011 [PMID: 21589862] (Mouse)
• Childs GV, Akhter N, Haney A The somatotrope as a metabolic sensor: deletion of leptin receptors causes obesity. Endocrinology, 2011;152(1):69-81. 2011 [PMID: 21084451] (Mouse)
• Wanderling S, Simen BB, Ostrovsky O, Ahmed NT, Vogen SM, Gidalevitz T, Argon Y GRP94 is essential for mesoderm induction and muscle development because it regulates insulin-like growth factor secretion. Mol. Biol. Cell, 2007;18(10):3764-75. 2007 [PMID: 17634284] (Mouse)
• Xiao Q, Zhao W, Beers DR, Yen AA, Xie W, Henkel JS, Appel SH Mutant SOD1(G93A) microglia are more neurotoxic relative to wild-type microglia. J. Neurochem., 2007;102(6):2008-19. 2007 [PMID: 17555556] (Mouse)
• Zhao W, Xie W, Xiao Q, Beers DR, Appel SH Protective effects of an anti-inflammatory cytokine, interleukin-4, on motoneuron toxicity induced by activated microglia. J. Neurochem., 2006;99(4):1176-87. 2006 [PMID: 17018025] (Rat)

Bioinformatics

• Gene Symbol: Igf1