Mouse IL-6R alpha Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse IL-6R alpha Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse IL-6 R alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-6 R alpha levels in cell culture supernates, serum, and heparin plasma. It contains NS0-expressed recombinant mouse IL-6 R alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-6 R alpha accurately. Results... obtained using natural mouse IL-6 R alpha showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6 R alpha .
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• Specificity: Natural and recombinant mouse IL-6 R alpha .
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: IL6R

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-6R alpha Quantikine ELISA Kit

• CD126
• gp80
• IL-6 R alpha
• IL6Q
• IL6R alpha
• IL-6R alpha
• IL6R
• IL-6R-1
• IL6RA
• IL-6Ra
• IL6RQ
• interleukin 6 receptor

Background

IL-6 R alpha is a transmembrane subunit of the receptor for Interleukin-6. It associates with gp130 which is a shared component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. IL-6 plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-6R alpha (MR600)(2)

Publications using MR600

• Milligan, C;Cowley, DO;Stewart, W;Curry, AM;Forbes, E;Rector, B;Hastie, A;Liu, L;Hawkins, GA; Enhanced Interleukin 6 Trans-Signaling Modulates Disease Process in Amyotrophic Lateral Sclerosis Mouse Models Brain sciences 2025-01-17 [PMID: 39851451] (Transgenic Mouse)
• EH Bent, LR Millán-Bar, I Zhuang, DR Goulet, J Fröse, MT Hemann Microenvironmental IL-6 inhibits anti-cancer immune responses generated by cytotoxic chemotherapy Nature Communications, 2021-10-28;12(1):6218. 2021-10-28 [PMID: 34711820] (Mouse, Transgenic Mouse)

FAQs for IL-6R alpha (MR600). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IL6R