Mouse IL-18 DuoSet ELISA, 5 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse IL-18 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-18 DuoSet ELISA, 5 Plate

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1 gamma , is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-gamma and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-gamma that inhibits IgE production.

Publications for IL-18/IL-1F4 (DY7625-05)(30)

Publications using DY7625-05

• Speaks, S;Papa, J;McFadden, M;Roettger, JE;Liu, BD;Mohan, S;Reznik, BM;Leumi, S;Cable, JM;Forero, A;Yount, JS; Gasdermin E is Dispensable for H1N1 Influenza Virus Pathogenesis in Mice bioRxiv : the preprint server for biology 2025-07-29 [PMID: 40766393] (Mouse)
• Li, Y;Xu, C;Liu, J;Guo, M;Wang, J;Bai, X;Cheng, Y;Luan, X;Pei, H;Zhang, C;Liu, H;Chen, M;Tang, B; Targeting the complement-mTOR-autophagy axis: the role of apolipoprotein E in depression BMC biology 2025-07-28 [PMID: 40722026] (Mouse)
• Yan, C;Lin, X;Guan, J;Ding, W;Yue, Z;Tang, Z;Meng, X;Zhao, B;Song, Z;Li, D;Jiang, T; SIRT3 Deficiency Promotes Lung Endothelial Pyroptosis Through Impairing Mitophagy to Activate NLRP3 Inflammasome During Sepsis-Induced Acute Lung Injury Molecular and cellular biology 2024-11-18 [PMID: 39556090] (Mouse)
• Wen, S;Arakawa, H;Yokoyama, S;Shirasaka, Y;Higashida, H;Tamai, I; Functional identification of soluble uric acid as an endogenous inhibitor of CD38 eLife 2024-11-11 [PMID: 39527634] (Mouse)
• Nabukhotna, K;Kordus, SL;Shupe, JA;Cano Rodríguez, R;Smith, A;Bohannon, JK;Washington, MK;Lacy, DB; Purified CDT toxins and a clean deletion within the CDT locus provide novel insights into the contribution of binary toxin in cellular inflammation and Clostridioides difficile infection PLoS pathogens 2024-09-19 [PMID: 39298531] (Mouse)
• Klein, R;Onyuru, J;Viera, EM;Putnam, CD;Hoffman, HM;Hastings, ML; Modulating NLRP3 splicing with antisense oligonucleotides to control pathological inflammation bioRxiv : the preprint server for biology 2024-09-06 [PMID: 39282364] (Mouse)
• Wu, X;Wang, Z;Croce, KR;Li, F;Cui, J;D'Agati, VD;Soni, RK;Khalid, S;Saleheen, D;Tabas, I;Yamamoto, A;Zhang, H; Macrophage WDFY3, a protector against autoimmunity bioRxiv : the preprint server for biology 2024-08-19 [PMID: 39229152] (Mouse)
• Pinzan, CF;Valero, C;de Castro, PA;da Silva, JL;Earle, K;Liu, H;Horta, MAC;Kniemeyer, O;Krüger, T;Pschibul, A;Cömert, DN;Heinekamp, T;Brakhage, AA;Steenwyk, JL;Mead, ME;Hermsdorf, N;Filler, SG;da Rosa-Garzon, NG;Delbaje, E;Bromley, MJ;Cabral, H;Diehl, C;Angeli, CB;Palmisano, G;Ibrahim, AS;Rinker, DC;Sauters, TJC;Steffen, K;Gumilang, A;Rokas, A;Gago, S;Dos Reis, TF;Goldman, GH; Aspergillus fumigatus conidial surface-associated proteome reveals factors for fungal evasion and host immunity modulation Nature microbiology 2024-08-27 [PMID: 39191887] (Mouse)
• Zhang, WY;Wei, QQ;Zhang, T;Wang, CS;Chen, J;Wang, JH;Xie, X;Jiang, P; Microglial AKAP8L: a key mediator in diabetes-associated cognitive impairment via autophagy inhibition and neuroinflammation triggering Journal of neuroinflammation 2024-07-20 [PMID: 39033121] (Mouse)
• Rizzo, GP;Sanches, RC;Chavero, C;Bianchi, DS;Apuzzo, E;Herrera, SE;Agazzi, ML;Keitelman, IA;Trevani, AS;Oliveira, SC;Azzaroni, O;Smaldini, PL;Docena, GH; Poly(allylamine)-tripolyphosphate polymeric nanoparticle as an NLRP3-dependent systemic adjuvant for the vaccine development bioRxiv : the preprint server for biology 2024-07-04 [PMID: 39005275] (Mouse)
• Wang, Y;Gao, JZ;Sakaguchi, T;Maretzky, T;Gurung, P;Narayanan, NS;Short, S;Xiong, Y;Kang, Z; LRRK2 G2019S Promotes Colon Cancer Potentially via LRRK2-GSDMD Axis-Mediated Gut Inflammation Cells 2024-03-23 [PMID: 38607004] (Transgenic Mouse)
• Tao, X;Xue, F;Xu, J;Wang, W; Platelet-rich plasma-derived extracellular vesicles inhibit NF-?B/NLRP3 pathway-mediated pyroptosis in intervertebral disc degeneration via the MALAT1/microRNA-217/SIRT1 axis Cellular signalling 2024-02-17 [PMID: 38373669] (Mouse)
• Kim, MY;Jeong, B;Lee, GS;Jeon, H;Yang, YM;Yang, H;Han, YH; Panaxydol extracted from Panax ginseng inhibits NLRP3 inflammasome activation to ameliorate NASH-induced liver injury International immunopharmacology 2024-01-22 [PMID: 38262161] (Mouse)
• Ayyubova, G;Kodali, M;Upadhya, R;Madhu, LN;Attaluri, S;Somayaji, Y;Shuai, B;Rao, S;Shankar, G;Shetty, AK; Extracellular vesicles from hiPSC-NSCs can prevent peripheral inflammation-induced cognitive dysfunction with inflammasome inhibition and improved neurogenesis in the hippocampus Journal of neuroinflammation 2023-12-12 [PMID: 38087314] (Mouse)
• Talley, S;Rademacher, DJ;Campbell, EM; Inflammasome activation occurs in CD4+ and CD8+ T cells during graft-versus-host disease Cell death & disease 2023-09-25 [PMID: 37749127] (Mouse)
• Yang, Y;Gao, ZF;Hou, GG;Meng, QG;Hou, Y; Discovery of anti-neuroinflammatory agents from 1,4,5,6-tetrahydrobenzo[2,3]oxepino[4,5-d]pyrimidin-2-amine derivatives by regulating microglia polarization European journal of medicinal chemistry 2023-11-05 [PMID: 37544188] (Mouse)
• Amo-Aparicio, J;Daly, J;Højen, JF;Dinarello, CA; Pharmacologic inhibition of NLRP3 reduces the levels of ?-synuclein and protects dopaminergic neurons in a model of Parkinson's disease Journal of neuroinflammation 2023-06-22 [PMID: 37349821] (Mouse)
• Guo, Y;Gu, D;Huang, T;Li, A;Zhou, Y;Kang, X;Meng, C;Xiong, D;Song, L;Jiao, X;Pan, Z; Salmonella Enteritidis T1SS protein SiiD inhibits NLRP3 inflammasome activation via repressing the mtROS-ASC dependent pathway PLoS pathogens 2023-05-08 [PMID: 37155697] (Transgenic Mouse, Mouse)
• Huang, P;Thomas, CC;Ambati, K;Dholkawala, R;Nagasaka, A;Yerramothu, P;Narendran, S;Pereira, F;Nagasaka, Y;Apicella, I;Cai, X;Makin, RD;Magagnoli, J;Stains, CI;Yin, R;Wang, SB;Gelfand, BD;Ambati, J; Kamuvudine-9 Protects Retinal Structure and Function in a Novel Model of Experimental Rhegmatogenous Retinal Detachment Investigative ophthalmology & visual science 2023-05-01 [PMID: 37129905] (Mouse)
• JT Clark, OE Weizman, DL Aldridge, LA Shallberg, J Eberhard, Z Lanzar, D Wasche, JD Huck, T Zhou, AM Ring, CA Hunter IL-18BP mediates the balance between protective and pathological immune responses to Toxoplasma gondii Cell Reports, 2023-02-23;42(3):112147. 2023-02-23 [PMID: 36827187] (Mouse)
• KK Jang, T Heaney, M London, Y Ding, F Yeung, D Ercelen, YH Chen, J Axelrad, S Gurunathan, A Marijke Ke, ME Griffin, HC Hang, K Cadwell Antimicrobial overproduction sustains intestinal inflammation by inhibiting Enterococcus colonization bioRxiv : the preprint server for biology, 2023-02-01;0(0):. 2023-02-01 [PMID: 36778381] (Mouse)
• S Singh, IA Shaikh, SS More, MH Mahnashi, HM Almohaimee, M El-Sherbin, MM Ghoneim, A Umar, HK Soni, H Agrawal, BA Mannasaheb, AA Khan, UM Muddapur, SMS Iqubal Blockage of KHSRP-NLRP3 by MCC950 Can Reverse the Effect of Manganese-Induced Neuroinflammation in N2a Cells and Rat Brain International Journal of Molecular Sciences, 2022-10-30;23(21):. 2022-10-30 [PMID: 36362011] (Mouse, Rat)
• Y Ni, GH Wu, JJ Cai, R Zhang, Y Zheng, JQ Liu, XH Yang, X Yang, Y Shen, JM Lai, XM Ye, SJ Mo Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation International journal of biological sciences, 2022-08-08;18(13):5168-5184. 2022-08-08 [PMID: 35982894] (Mouse)
• I Cho, BN Koo, SY Kim, S Park, EJ Kim, EH Kam, J Kim Neuroprotective Effect of Dexmedetomidine against Postoperative Cognitive Decline via NLRP3 Inflammasome Signaling Pathway International Journal of Molecular Sciences, 2022-08-08;23(15):. 2022-08-08 [PMID: 35955939] (Mouse)
• ZZ Zhang, SY Xian, C Bao, F Chen MicroRNA-299a-5p Protects against Spinal Cord Injury through Activating AMPK Pathway Oxidative Medicine and Cellular Longevity, 2022-05-13;2022(0):8659587. 2022-05-13 [PMID: 35602094] (Mouse)
• K Peukert, F Steinhagen, M Fox, C Feuerborn, S Schulz, B Seeliger, P Schuss, M Schneider, S Frede, A Sauer, C Putensen, E Latz, C Wilhelm, C Bode Tetracycline ameliorates silica-induced pulmonary inflammation and fibrosis via inhibition of caspase-1 Respiratory Research, 2022-02-07;23(1):21. 2022-02-07 [PMID: 35130879] (Mouse)
• M Mae, MI Alam, Y Yamashita, Y Ozaki, K Higuchi, SM Ziauddin, JL Montenegro, E Sakai, T Tsukuba, A Yoshimura The Role of Cytokines Produced via the NLRP3 Inflammasome in Mouse Macrophages Stimulated with Dental Calculus in Osteoclastogenesis International Journal of Molecular Sciences, 2021-11-18;22(22):. 2021-11-18 [PMID: 34830316] (Mouse)
• S Rastogi, S Ellinwood, J Augenstrei, KD Mayer-Barb, V Briken Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF PloS Pathogens, 2021-07-29;17(7):e1009712. 2021-07-29 [PMID: 34324582] (Mouse)
• J Xing, X Zhou, M Fang, E Zhang, LJ Minze, Z Zhang DHX15 is required to control RNA virus-induced intestinal inflammation Cell Reports, 2021-06-22;35(12):109205. 2021-06-22 [PMID: 34161762] (Mouse)
• FRC Costa, JA Leite, DM Rassi, JF da Silva, J Elias-Oliv, JB Guimarães, MC Foss-Freit, NOS Câmara, A Pontillo, RC Tostes, JS Silva, D Carlos NLRP1 acts as a negative regulator of Th17 cell programming in mice and humans with autoimmune diabetes Cell Reports, 2021-05-25;35(8):109176. 2021-05-25 [PMID: 34038731] (Mouse)

FAQs for IL-18/IL-1F4 (DY7625-05). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.