Mouse IL-18 DuoSet ELISA, 5 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse IL-18 DuoSet ELISA, 5 Plate Summary

• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-18 DuoSet ELISA, 5 Plate

• Iboctadekin
• IFN-gamma-inducing factor
• IGIF
• IGIFIL-1 gamma
• IL18
• IL-18
• IL-18MGC12320
• IL-1F4
• IL1F4iboctadekin
• IL-1g
• Interferon gamma-inducing factor
• interleukin 18 (interferon-gamma-inducing factor)
• Interleukin-1 gamma
• interleukin-18

Background

Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1 gamma , is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-gamma and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-gamma that inhibits IgE production.

Publications for IL-18/IL-1F4 (DY7625-05)(32)

Publications using DY7625-05

• Calvo Alvarez, E;Sfogliarini, C;La Rosa, F;Saresella, M;Dolci, M;Vegeto, E;Taramelli, D;Basilico, N;Clerici, M; Immune subversion by Leishmania infantum parasites suppresses NLRP3-driven inflammatory responses in amyloid-?-activated microglia Journal of neuroinflammation 2025-10-29 [PMID: 41162989] (Mouse)
• Wu, X;Wang, Z;Croce, KR;Li, F;Cui, J;D'Agati, VD;Soni, RK;Tabas, I;Yamamoto, A;Zhang, H; Macrophage WDFY3 mitigates autoimmunity by enhancing efferocytosis and suppressing T cell activation in mice Nature communications 2025-09-30 [PMID: 41027899] (Mouse)
• Speaks, S;Papa, J;McFadden, M;Roettger, JE;Liu, BD;Mohan, S;Reznik, BM;Leumi, S;Cable, JM;Forero, A;Yount, JS; Gasdermin E is Dispensable for H1N1 Influenza Virus Pathogenesis in Mice bioRxiv : the preprint server for biology 2025-07-29 [PMID: 40766393] (Mouse)
• Li, Y;Xu, C;Liu, J;Guo, M;Wang, J;Bai, X;Cheng, Y;Luan, X;Pei, H;Zhang, C;Liu, H;Chen, M;Tang, B; Targeting the complement-mTOR-autophagy axis: the role of apolipoprotein E in depression BMC biology 2025-07-28 [PMID: 40722026] (Mouse)
• Yan, C;Lin, X;Guan, J;Ding, W;Yue, Z;Tang, Z;Meng, X;Zhao, B;Song, Z;Li, D;Jiang, T; SIRT3 Deficiency Promotes Lung Endothelial Pyroptosis Through Impairing Mitophagy to Activate NLRP3 Inflammasome During Sepsis-Induced Acute Lung Injury Molecular and cellular biology 2024-11-18 [PMID: 39556090] (Mouse)
• Wen, S;Arakawa, H;Yokoyama, S;Shirasaka, Y;Higashida, H;Tamai, I; Functional identification of soluble uric acid as an endogenous inhibitor of CD38 eLife 2024-11-11 [PMID: 39527634] (Mouse)
• Nabukhotna, K;Kordus, SL;Shupe, JA;Cano Rodríguez, R;Smith, A;Bohannon, JK;Washington, MK;Lacy, DB; Purified CDT toxins and a clean deletion within the CDT locus provide novel insights into the contribution of binary toxin in cellular inflammation and Clostridioides difficile infection PLoS pathogens 2024-09-19 [PMID: 39298531] (Mouse)
• Klein, R;Onyuru, J;Viera, EM;Putnam, CD;Hoffman, HM;Hastings, ML; Modulating NLRP3 splicing with antisense oligonucleotides to control pathological inflammation bioRxiv : the preprint server for biology 2024-09-06 [PMID: 39282364] (Mouse)
• Wu, X;Wang, Z;Croce, KR;Li, F;Cui, J;D'Agati, VD;Soni, RK;Khalid, S;Saleheen, D;Tabas, I;Yamamoto, A;Zhang, H; Macrophage WDFY3, a protector against autoimmunity bioRxiv : the preprint server for biology 2024-08-19 [PMID: 39229152] (Mouse)
• Pinzan, CF;Valero, C;de Castro, PA;da Silva, JL;Earle, K;Liu, H;Horta, MAC;Kniemeyer, O;Krüger, T;Pschibul, A;Cömert, DN;Heinekamp, T;Brakhage, AA;Steenwyk, JL;Mead, ME;Hermsdorf, N;Filler, SG;da Rosa-Garzon, NG;Delbaje, E;Bromley, MJ;Cabral, H;Diehl, C;Angeli, CB;Palmisano, G;Ibrahim, AS;Rinker, DC;Sauters, TJC;Steffen, K;Gumilang, A;Rokas, A;Gago, S;Dos Reis, TF;Goldman, GH; Aspergillus fumigatus conidial surface-associated proteome reveals factors for fungal evasion and host immunity modulation Nature microbiology 2024-08-27 [PMID: 39191887] (Mouse)
• Zhang, WY;Wei, QQ;Zhang, T;Wang, CS;Chen, J;Wang, JH;Xie, X;Jiang, P; Microglial AKAP8L: a key mediator in diabetes-associated cognitive impairment via autophagy inhibition and neuroinflammation triggering Journal of neuroinflammation 2024-07-20 [PMID: 39033121] (Mouse)
• Rizzo, GP;Sanches, RC;Chavero, C;Bianchi, DS;Apuzzo, E;Herrera, SE;Agazzi, ML;Keitelman, IA;Trevani, AS;Oliveira, SC;Azzaroni, O;Smaldini, PL;Docena, GH; Poly(allylamine)-tripolyphosphate polymeric nanoparticle as an NLRP3-dependent systemic adjuvant for the vaccine development bioRxiv : the preprint server for biology 2024-07-04 [PMID: 39005275] (Mouse)
• Wang, Y;Gao, JZ;Sakaguchi, T;Maretzky, T;Gurung, P;Narayanan, NS;Short, S;Xiong, Y;Kang, Z; LRRK2 G2019S Promotes Colon Cancer Potentially via LRRK2-GSDMD Axis-Mediated Gut Inflammation Cells 2024-03-23 [PMID: 38607004] (Transgenic Mouse)
• Tao, X;Xue, F;Xu, J;Wang, W; Platelet-rich plasma-derived extracellular vesicles inhibit NF-?B/NLRP3 pathway-mediated pyroptosis in intervertebral disc degeneration via the MALAT1/microRNA-217/SIRT1 axis Cellular signalling 2024-02-17 [PMID: 38373669] (Mouse)
• Kim, MY;Jeong, B;Lee, GS;Jeon, H;Yang, YM;Yang, H;Han, YH; Panaxydol extracted from Panax ginseng inhibits NLRP3 inflammasome activation to ameliorate NASH-induced liver injury International immunopharmacology 2024-01-22 [PMID: 38262161] (Mouse)
• Ayyubova, G;Kodali, M;Upadhya, R;Madhu, LN;Attaluri, S;Somayaji, Y;Shuai, B;Rao, S;Shankar, G;Shetty, AK; Extracellular vesicles from hiPSC-NSCs can prevent peripheral inflammation-induced cognitive dysfunction with inflammasome inhibition and improved neurogenesis in the hippocampus Journal of neuroinflammation 2023-12-12 [PMID: 38087314] (Mouse)
• Talley, S;Rademacher, DJ;Campbell, EM; Inflammasome activation occurs in CD4+ and CD8+ T cells during graft-versus-host disease Cell death & disease 2023-09-25 [PMID: 37749127] (Mouse)
• Yang, Y;Gao, ZF;Hou, GG;Meng, QG;Hou, Y; Discovery of anti-neuroinflammatory agents from 1,4,5,6-tetrahydrobenzo[2,3]oxepino[4,5-d]pyrimidin-2-amine derivatives by regulating microglia polarization European journal of medicinal chemistry 2023-11-05 [PMID: 37544188] (Mouse)
• Amo-Aparicio, J;Daly, J;Højen, JF;Dinarello, CA; Pharmacologic inhibition of NLRP3 reduces the levels of ?-synuclein and protects dopaminergic neurons in a model of Parkinson's disease Journal of neuroinflammation 2023-06-22 [PMID: 37349821] (Mouse)
• Guo, Y;Gu, D;Huang, T;Li, A;Zhou, Y;Kang, X;Meng, C;Xiong, D;Song, L;Jiao, X;Pan, Z; Salmonella Enteritidis T1SS protein SiiD inhibits NLRP3 inflammasome activation via repressing the mtROS-ASC dependent pathway PLoS pathogens 2023-05-08 [PMID: 37155697] (Transgenic Mouse, Mouse)
• Huang, P;Thomas, CC;Ambati, K;Dholkawala, R;Nagasaka, A;Yerramothu, P;Narendran, S;Pereira, F;Nagasaka, Y;Apicella, I;Cai, X;Makin, RD;Magagnoli, J;Stains, CI;Yin, R;Wang, SB;Gelfand, BD;Ambati, J; Kamuvudine-9 Protects Retinal Structure and Function in a Novel Model of Experimental Rhegmatogenous Retinal Detachment Investigative ophthalmology & visual science 2023-05-01 [PMID: 37129905] (Mouse)
• JT Clark, OE Weizman, DL Aldridge, LA Shallberg, J Eberhard, Z Lanzar, D Wasche, JD Huck, T Zhou, AM Ring, CA Hunter IL-18BP mediates the balance between protective and pathological immune responses to Toxoplasma gondii Cell Reports, 2023-02-23;42(3):112147. 2023-02-23 [PMID: 36827187] (Mouse)
• KK Jang, T Heaney, M London, Y Ding, F Yeung, D Ercelen, YH Chen, J Axelrad, S Gurunathan, A Marijke Ke, ME Griffin, HC Hang, K Cadwell Antimicrobial overproduction sustains intestinal inflammation by inhibiting Enterococcus colonization bioRxiv : the preprint server for biology, 2023-02-01;0(0):. 2023-02-01 [PMID: 36778381] (Mouse)
• S Singh, IA Shaikh, SS More, MH Mahnashi, HM Almohaimee, M El-Sherbin, MM Ghoneim, A Umar, HK Soni, H Agrawal, BA Mannasaheb, AA Khan, UM Muddapur, SMS Iqubal Blockage of KHSRP-NLRP3 by MCC950 Can Reverse the Effect of Manganese-Induced Neuroinflammation in N2a Cells and Rat Brain International Journal of Molecular Sciences, 2022-10-30;23(21):. 2022-10-30 [PMID: 36362011] (Mouse, Rat)
• Y Ni, GH Wu, JJ Cai, R Zhang, Y Zheng, JQ Liu, XH Yang, X Yang, Y Shen, JM Lai, XM Ye, SJ Mo Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation International journal of biological sciences, 2022-08-08;18(13):5168-5184. 2022-08-08 [PMID: 35982894] (Mouse)
• I Cho, BN Koo, SY Kim, S Park, EJ Kim, EH Kam, J Kim Neuroprotective Effect of Dexmedetomidine against Postoperative Cognitive Decline via NLRP3 Inflammasome Signaling Pathway International Journal of Molecular Sciences, 2022-08-08;23(15):. 2022-08-08 [PMID: 35955939] (Mouse)
• ZZ Zhang, SY Xian, C Bao, F Chen MicroRNA-299a-5p Protects against Spinal Cord Injury through Activating AMPK Pathway Oxidative Medicine and Cellular Longevity, 2022-05-13;2022(0):8659587. 2022-05-13 [PMID: 35602094] (Mouse)
• K Peukert, F Steinhagen, M Fox, C Feuerborn, S Schulz, B Seeliger, P Schuss, M Schneider, S Frede, A Sauer, C Putensen, E Latz, C Wilhelm, C Bode Tetracycline ameliorates silica-induced pulmonary inflammation and fibrosis via inhibition of caspase-1 Respiratory Research, 2022-02-07;23(1):21. 2022-02-07 [PMID: 35130879] (Mouse)
• M Mae, MI Alam, Y Yamashita, Y Ozaki, K Higuchi, SM Ziauddin, JL Montenegro, E Sakai, T Tsukuba, A Yoshimura The Role of Cytokines Produced via the NLRP3 Inflammasome in Mouse Macrophages Stimulated with Dental Calculus in Osteoclastogenesis International Journal of Molecular Sciences, 2021-11-18;22(22):. 2021-11-18 [PMID: 34830316] (Mouse)
• S Rastogi, S Ellinwood, J Augenstrei, KD Mayer-Barb, V Briken Mycobacterium tuberculosis inhibits the NLRP3 inflammasome activation via its phosphokinase PknF PloS Pathogens, 2021-07-29;17(7):e1009712. 2021-07-29 [PMID: 34324582] (Mouse)
• J Xing, X Zhou, M Fang, E Zhang, LJ Minze, Z Zhang DHX15 is required to control RNA virus-induced intestinal inflammation Cell Reports, 2021-06-22;35(12):109205. 2021-06-22 [PMID: 34161762] (Mouse)
• FRC Costa, JA Leite, DM Rassi, JF da Silva, J Elias-Oliv, JB Guimarães, MC Foss-Freit, NOS Câmara, A Pontillo, RC Tostes, JS Silva, D Carlos NLRP1 acts as a negative regulator of Th17 cell programming in mice and humans with autoimmune diabetes Cell Reports, 2021-05-25;35(8):109176. 2021-05-25 [PMID: 34038731] (Mouse)

FAQs for IL-18/IL-1F4 (DY7625-05). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.