Mouse IL-12 p40 NonAllele-specific Quantikine ELISA, 2 Plate



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Applications ELISA

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Mouse IL-12 p40 NonAllele-specific Quantikine ELISA, 2 Plate Summary

The Quantikine Mouse IL-12/IL-23 p40 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse p40 subunit in IL-12 p70, IL-23, p40 homodimer, and p40 monomer in cell culture supernates, mouse serum, and plasma. It contains recombinant mouse IL-12/23 p40 homodimer and antibodies raised against the p40 subunit of recombinant mouse IL-12 p70. This immunoassay has been shown to accu...rately quantitate the recombinant mouse IL-12/IL-23 p40. Results obtained using natural mouse IL-12/IL-23 p40 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12/IL-23 p40.
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Natural and recombinant mouse IL-12/IL-23 p40
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using MP400.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-12 p40 NonAllele-specific Quantikine ELISA, 2 Plate

  • CLMF p40
  • CLMF
  • CLMF2
  • Cytotoxic lymphocyte maturation factor 40 kDa subunit
  • IL12 p40
  • IL-12 p40
  • IL-12 subunit p40
  • IL12B
  • IL-12B
  • IL-12BNK cell stimulatory factor chain 2
  • interleukin 12, p40
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
  • interleukin-12 beta chain
  • interleukin-12 subunit beta
  • natural killer cell stimulatory factor, 40 kD subunit
  • NKSF
  • NKSF2
  • NKSF2IL12, subunit p40


Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for IL-12/IL-23 p40 (MP400)(16)

We have publications tested in 1 confirmed species: Mouse.

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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using MP400 Applications Species
Ylostalo J, Bartosh T, Coble K, Prockop D Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype. Stem Cells, 2012;30(10):2283-96. 2012 [PMID: 22865689] (Mouse) Mouse
Gais P, Reim D, Jusek G, Rossmann-Bloeck T, Weighardt H, Pfeffer K, Altmayr F, Janssen KP, Holzmann B Cutting edge: Divergent cell-specific functions of MyD88 for inflammatory responses and organ injury in septic peritonitis. J. Immunol., 2012;188(12):5833-7. 2012 [PMID: 22586041] (Mouse) Mouse
Gottfries J, Melgar S, Michaelsson E Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease. PLoS ONE, 2012;7(1):e30005. 2012 [PMID: 22279558] (Mouse) Mouse
Bondue B, Vosters O, de Nadai P, Glineur S, De Henau O, Luangsay S, Van Gool F, Communi D, De Vuyst P, Desmecht D, Parmentier M ChemR23 dampens lung inflammation and enhances anti-viral immunity in a mouse model of acute viral pneumonia. PLoS Pathog., 2011;7(11):e1002358. 2011 [PMID: 22072972] (Mouse) Mouse
Li N, Ghia JE, Wang H, McClemens J, Cote F, Suehiro Y, Mallet J, Khan WI Serotonin activates dendritic cell function in the context of gut inflammation. Am. J. Pathol., 2011;178(2):662-71. 2011 [PMID: 21281798] (Mouse) Mouse
Fukui R, Saitoh S, Matsumoto F, Kozuka-Hata H, Oyama M, Tabeta K, Beutler B, Miyake K Unc93B1 biases Toll-like receptor responses to nucleic acid in dendritic cells toward DNA- but against RNA-sensing. J. Exp. Med., 2009;206(6):1339-50. 2009 [PMID: 19451267] (Mouse) Mouse
Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia. Am. J. Pathol., 2009;174(1):153-63. 2009 [PMID: 19095958] (Mouse) Mouse
Divangahi M, Mostowy S, Coulombe F, Kozak R, Guillot L, Veyrier F, Kobayashi KS, Flavell RA, Gros P, Behr MA NOD2-deficient mice have impaired resistance to Mycobacterium tuberculosis infection through defective innate and adaptive immunity. J. Immunol., 2008;181(10):7157-65. 2008 [PMID: 18981137] (Mouse) Mouse
Li Q, Guo Z, Xu X, Xia S, Cao X Pulmonary stromal cells induce the generation of regulatory DC attenuating T-cell-mediated lung inflammation. Eur. J. Immunol., 2008;38(10):2751-61. 2008 [PMID: 18825748] (Mouse) Mouse
Kang MJ, Lee CG, Lee JY, Dela Cruz CS, Chen ZJ, Enelow R, Elias JA Cigarette smoke selectively enhances viral PAMP- and virus-induced pulmonary innate immune and remodeling responses in mice. J. Clin. Invest., 2008;118(8):2771-84. 2008 [PMID: 18654661] (Mouse) Mouse
Show All 16 Publications.

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FAQs for IL-12/IL-23 p40 (MP400). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Gene Symbol Il12b