Mouse IL-12 p40 NonAllele-specific Quantikine ELISA, 2 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse IL-12 p40 NonAllele-specific Quantikine ELISA, 2 Plate Summary

• Background: The Quantikine Mouse IL-12/IL-23 p40 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse p40 subunit in IL-12 p70, IL-23, p40 homodimer, and p40 monomer in cell culture supernates, mouse serum, and plasma. It contains recombinant mouse IL-12/23 p40 homodimer and antibodies raised against the p40 subunit of recombinant mouse IL-12 p70. This immunoassay has been shown to accu...rately quantitate the recombinant mouse IL-12/IL-23 p40. Results obtained using natural mouse IL-12/IL-23 p40 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12/IL-23 p40.
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• Specificity: Natural and recombinant mouse IL-12/IL-23 p40
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il12b

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-12 p40 NonAllele-specific Quantikine ELISA, 2 Plate

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-12/IL-23 p40 (MP400)(17)

Publications using MP400

• N Hagan, JL Kane, D Grover, L Woodworth, C Madore, J Saleh, J Sancho, J Liu, Y Li, J Proto, M Zelic, A Mahan, M Kothe, AA Scholte, M Fitzgerald, B Gisevius, A Haghikia, O Butovsky, D Ofengeim CSF1R signaling is a regulator of pathogenesis in progressive MS Cell Death Dis, 2020-10-23;11(10):904. 2020-10-23 [PMID: 33097690] (Mouse)
• Ylostalo J, Bartosh T, Coble K, Prockop D Human mesenchymal stem/stromal cells cultured as spheroids are self-activated to produce prostaglandin E2 that directs stimulated macrophages into an anti-inflammatory phenotype. Stem Cells, 2012-10-01;30(10):2283-96. 2012-10-01 [PMID: 22865689] (Mouse)
• Gais P, Reim D, Jusek G, Rossmann-Bloeck T, Weighardt H, Pfeffer K, Altmayr F, Janssen KP, Holzmann B Cutting edge: Divergent cell-specific functions of MyD88 for inflammatory responses and organ injury in septic peritonitis. J. Immunol., 2012-05-14;188(12):5833-7. 2012-05-14 [PMID: 22586041] (Mouse)
• Gottfries J, Melgar S, Michaelsson E Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease. PLoS ONE, 2012-01-18;7(1):e30005. 2012-01-18 [PMID: 22279558] (Mouse)
• Bondue B, Vosters O, de Nadai P, Glineur S, De Henau O, Luangsay S, Van Gool F, Communi D, De Vuyst P, Desmecht D, Parmentier M ChemR23 dampens lung inflammation and enhances anti-viral immunity in a mouse model of acute viral pneumonia. PLoS Pathog., 2011-11-03;7(11):e1002358. 2011-11-03 [PMID: 22072972] (Mouse)
• Li N, Ghia JE, Wang H, McClemens J, Cote F, Suehiro Y, Mallet J, Khan WI Serotonin activates dendritic cell function in the context of gut inflammation. Am. J. Pathol., 2011-02-01;178(2):662-71. 2011-02-01 [PMID: 21281798] (Mouse)
• Fukui R, Saitoh S, Matsumoto F, Kozuka-Hata H, Oyama M, Tabeta K, Beutler B, Miyake K Unc93B1 biases Toll-like receptor responses to nucleic acid in dendritic cells toward DNA- but against RNA-sensing. J. Exp. Med., 2009-05-18;206(6):1339-50. 2009-05-18 [PMID: 19451267] (Mouse)
• Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia. Am. J. Pathol., 2008-12-18;174(1):153-63. 2008-12-18 [PMID: 19095958] (Mouse)
• Divangahi M, Mostowy S, Coulombe F, Kozak R, Guillot L, Veyrier F, Kobayashi KS, Flavell RA, Gros P, Behr MA NOD2-deficient mice have impaired resistance to Mycobacterium tuberculosis infection through defective innate and adaptive immunity. J. Immunol., 2008-11-15;181(10):7157-65. 2008-11-15 [PMID: 18981137] (Mouse)
• Li Q, Guo Z, Xu X, Xia S, Cao X Pulmonary stromal cells induce the generation of regulatory DC attenuating T-cell-mediated lung inflammation. Eur. J. Immunol., 2008-10-01;38(10):2751-61. 2008-10-01 [PMID: 18825748] (Mouse)
• Kang MJ, Lee CG, Lee JY, Dela Cruz CS, Chen ZJ, Enelow R, Elias JA Cigarette smoke selectively enhances viral PAMP- and virus-induced pulmonary innate immune and remodeling responses in mice. J. Clin. Invest., 2008-08-01;118(8):2771-84. 2008-08-01 [PMID: 18654661] (Mouse)
• Yamazaki T, Nagata K, Kobayashi Y Cytokine production by M-CSF- and GM-CSF-induced mouse bone marrow-derived macrophages upon coculturing with late apoptotic cells. Cell. Immunol., 2008-06-03;251(2):124-30. 2008-06-03 [PMID: 18511028] (Mouse)
• Vanhoutte F, Paget C, Breuilh L, Fontaine J, Vendeville C, Goriely S, Ryffel B, Faveeuw C, Trottein F Toll-like receptor (TLR)2 and TLR3 synergy and cross-inhibition in murine myeloid dendritic cells. Immunol. Lett., 2007-12-17;116(1):86-94. 2007-12-17 [PMID: 18166232] (Mouse)
• Takahashi N, Kitazawa H, Iwabuchi N, Xiao JZ, Miyaji K, Iwatsuki K, Saito T Oral administration of an immunostimulatory DNA sequence from Bifidobacterium longum improves Th1/Th2 balance in a murine model. Biosci. Biotechnol. Biochem., 2006-08-01;70(8):2013-7. 2006-08-01 [PMID: 16926520] (Mouse)
• Burch LH, Yang IV, Whitehead GS, Chao FG, Berman KG, Schwartz DA The transcriptional response to lipopolysaccharide reveals a role for interferon-gamma in lung neutrophil recruitment. Am. J. Physiol. Lung Cell Mol. Physiol., 2006-06-09;291(4):L677-82. 2006-06-09 [PMID: 16766576] (Mouse)
• Santel A, Aleku M, Keil O, Endruschat J, Esche V, Fisch G, Dames S, Loffler K, Fechtner M, Arnold W, Giese K, Klippel A, Kaufmann J A novel siRNA-lipoplex technology for RNA interference in the mouse vascular endothelium. Gene Ther., 2006-04-20;13(16):1222-34. 2006-04-20 [PMID: 16625243] (Mouse)
• Bouis DA, Popova TG, Takashima A, Norgard MV Dendritic cells phagocytose and are activated by Treponema pallidum. Infect. Immun., 2001-01-01;69(1):518-28. 2001-01-01 [PMID: 11119545] (Mouse)

FAQs for IL-12/IL-23 p40 (MP400). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Il12b