Mouse IL-12 p40 Allele-specific Quantikine ELISA Kt, 2 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse IL-12 p40 Allele-specific Quantikine ELISA Kt, 2 Plate Summary

• Background: The Quantikine Mouse IL-12/IL-23 p40 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse p40 subunit in IL-12 p70, IL-23, p40 homodimer, and p40 monomer in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 p40 homodimer and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mo...use IL-12/IL-23 p40. Results obtained using natural mouse IL-12/IL-23 p40 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12/IL-23 p40.
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• Specificity: Natural and recombinant mouse IL-12/IL-23 p40
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il12b

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-12 p40 Allele-specific Quantikine ELISA Kt, 2 Plate

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-12/IL-23 p40 (M1240)(31)

Publications using M1240

• D Wang, Y Lin, F Xu, H Zhang, X Zhu, Z Liu, Y Hu, G Dong, B Sun, Y Yu, G Ma, Z Tang, D Legarda, A Ting, Y Liu, J Hou, L Dong, H Xiong SIRPalpha maintains macrophage homeostasis by interacting with PTK2B kinase in Mycobacterium tuberculosis infection and through autophagy and necroptosis EBioMedicine, 2022-10-03;85(0):104278. 2022-10-03 [PMID: 36202053] (Mouse)
• Katherine M. Still, Samantha J. Batista, Carleigh A. O’Brien, Oyebola O. Oyesola, Simon P. Früh, Lauren M. Webb et al. Astrocytes promote a protective immune response to brain Toxoplasma gondii infection via IL-33-ST2 signaling PLOS Pathogens 2020-10-27 [PMID: 33108405] (Mouse)
• T Kawabe, J Yi, A Kawajiri, K Hilligan, D Fang, N Ishii, H Yamane, J Zhu, D Jankovic, KS Kim, G Trinchieri, A Sher Requirements for the differentiation of innate T-bethigh memory-phenotype CD4+ T lymphocytes under steady state Nat Commun, 2020-07-06;11(1):3366. 2020-07-06 [PMID: 32632165] (Mouse)
• M Nagaoka, T Yashiro, Y Uchida, T Ando, M Hara, H Arai, H Ogawa, K Okumura, K Kasakura, C Nishiyama The Orphan Nuclear Receptor NR4A3 Is Involved in the Function of Dendritic Cells J. Immunol., 2017-09-11;0(0):. 2017-09-11 [PMID: 28893954] (Mouse)
• Filardy AA, Pires DR, Nunes MP, Takiya CM, Freire-de-Lima CG, Ribeiro-Gomes FL, DosReis GA Proinflammatory clearance of apoptotic neutrophils induces an IL-12(low)IL-10(high) regulatory phenotype in macrophages. J. Immunol., 2010-07-21;185(4):2044-50. 2010-07-21 [PMID: 20660352] (Mouse)
• Watanabe T, Asano N, Fichtner-Feigl S, Gorelick PL, Tsuji Y, Matsumoto Y, Chiba T, Fuss IJ, Kitani A, Strober W NOD1 contributes to mouse host defense against Helicobacter pylori via induction of type I IFN and activation of the ISGF3 signaling pathway. J. Clin. Invest., 2010-04-12;120(5):1645-62. 2010-04-12 [PMID: 20389019] (Mouse)
• Dorhoi A, Desel C, Yeremeev V, Pradl L, Brinkmann V, Mollenkopf HJ, Hanke K, Gross O, Ruland J, Kaufmann SH The adaptor molecule CARD9 is essential for tuberculosis control. J. Exp. Med., 2010-03-29;207(4):777-92. 2010-03-29 [PMID: 20351059] (Mouse)
• Ghiringhelli F, Apetoh L, Tesniere A, Aymeric L, Ma Y, Ortiz C, Vermaelen K, Panaretakis T, Mignot G, Ullrich E, Perfettini JL, Schlemmer F, Tasdemir E, Uhl M, Genin P, Civas A, Ryffel B, Kanellopoulos J, Tschopp J, Andre F, Lidereau R, McLaughlin NM, Haynes NM, Smyth MJ, Kroemer G, Zitvogel L Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors. Nat. Med., 2009-09-20;15(10):1170-8. 2009-09-20 [PMID: 19767732] (Mouse)
• Vultaggio A, Nencini F, Fitch PM, Fili L, Maggi L, Fanti P, deVries A, Beccastrini E, Palandri F, Manuelli C, Bani D, Giudizi MG, Guarna A, Annunziato F, Romagnani S, Maggi E, Howie SE, Parronchi P Modified adenine (9-benzyl-2-butoxy-8-hydroxyadenine) redirects Th2-mediated murine lung inflammation by triggering TLR7. J. Immunol., 2009-01-15;182(2):880-9. 2009-01-15 [PMID: 19124731] (Mouse)
• Da Silva CA, Hartl D, Liu W, Lee CG, Elias JA TLR-2 and IL-17A in chitin-induced macrophage activation and acute inflammation. J. Immunol., 2008-09-15;181(6):4279-86. 2008-09-15 [PMID: 18768886] (Mouse)
• Olleros ML, Martin ML, Vesin D, Fotio AL, Santiago-Raber ML, Rubbia-Brandt L, Spahr L, Hadengue A, Garcia I Fat diet and alcohol-induced steatohepatitis after LPS challenge in mice: role of bioactive TNF and Th1 type cytokines. Cytokine, 2008-08-22;44(1):118-25. 2008-08-22 [PMID: 18722787] (Mouse)
• Voskas D, Babichev Y, Ling LS, Alami J, Shaked Y, Kerbel RS, Ciruna B, Dumont DJ An eosinophil immune response characterizes the inflammatory skin disease observed in Tie-2 transgenic mice. J. Leukoc. Biol., 2008-05-01;84(1):59-67. 2008-05-01 [PMID: 18443190] (Mouse)
• Littlejohn JE, Cao X, Miller SD, Ozvaran MK, Jupiter D, Zhang L, Rodarte C, Smythe WR Bcl-xL antisense oligonucleotide and cisplatin combination therapy extends survival in SCID mice with established mesothelioma xenografts. Int. J. Cancer, 2008-07-01;123(1):202-8. 2008-07-01 [PMID: 18360826] (Mouse)
• Kelschenbach J, Ninkovic J, Wang J, Krishnan A, Charboneau R, Barke RA, Roy S Morphine withdrawal inhibits IL-12 induction in a macrophage cell line through a mechanism that involves cAMP. J. Immunol., 2008-03-15;180(6):3670-9. 2008-03-15 [PMID: 18322172] (Mouse)
• Kaczorowski DJ, Nakao A, Mollen KP, Vallabhaneni R, Sugimoto R, Kohmoto J, Tobita K, Zuckerbraun BS, McCurry KR, Murase N, Billiar TR Toll-like receptor 4 mediates the early inflammatory response after cold ischemia/reperfusion. Transplantation, 2007-11-27;84(10):1279-87. 2007-11-27 [PMID: 18049113] (Mouse)
• Vogl T, Tenbrock K, Ludwig S, Leukert N, Ehrhardt C, van Zoelen MA, Nacken W, Foell D, van der Poll T, Sorg C, Roth J Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting lethal, endotoxin-induced shock. Nat. Med., 2007-09-02;13(9):1042-9. 2007-09-02 [PMID: 17767165] (Mouse)
• Fujita H, Asahina A, Komine M, Tamaki K The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Cell. Immunol., 2007-05-15;245(2):70-9. 2007-05-15 [PMID: 17507001] (Mouse)
• Kurooka M, Kaneda Y Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells. Cancer Res., 2007-01-01;67(1):227-36. 2007-01-01 [PMID: 17210703] (Mouse)
• Bullard DC, Hu X, Schoeb TR, Collins RG, Beaudet AL, Barnum SR Intercellular adhesion molecule-1 expression is required on multiple cell types for the development of experimental autoimmune encephalomyelitis. J. Immunol., 2007-01-15;178(2):851-7. 2007-01-15 [PMID: 17202346] (Mouse)
• Furuta T, Yoshikawa Y Roles of the small intestine for induction of toll-like receptor 4-mediated innate resistance in naturally acquired murine toxoplasmosis. Int. Immunol., 2006-10-11;18(12):1655-62. 2006-10-11 [PMID: 17035347] (Mouse)
• Deng L, Dai P, Ding W, Granstein RD, Shuman S Vaccinia virus infection attenuates innate immune responses and antigen presentation by epidermal dendritic cells. J. Virol., 2006-10-01;80(20):9977-87. 2006-10-01 [PMID: 17005676] (Mouse)
• Grover A, Ahmed MF, Singh B, Verma I, Sharma P, Khuller GK A multivalent combination of experimental antituberculosis DNA vaccines based on Ag85B and regions of difference antigens. Microbes Infect., 2006-07-18;8(9):2390-9. 2006-07-18 [PMID: 16962360] (Mouse)
• Wang ZY, Yang D, Chen Q, Leifer CA, Segal DM, Su SB, Caspi RR, Howard ZO, Oppenheim JJ Induction of dendritic cell maturation by pertussis toxin and its B subunit differentially initiate Toll-like receptor 4-dependent signal transduction pathways. Exp. Hematol., 2006-08-01;34(8):1115-24. 2006-08-01 [PMID: 16863919] (Mouse)
• Weijer S, Florquin S, van der Poll T Endogenous interleukin-12 improves the early antimicrobial host response to murine Escherichia coli peritonitis. Shock, 2005-01-01;23(1):54-8. 2005-01-01 [PMID: 15614132] (Mouse)
• Lukashev D, Ohta A, Apasov S, Chen JF, Sitkovsky M Cutting edge: Physiologic attenuation of proinflammatory transcription by the Gs protein-coupled A2A adenosine receptor in vivo. J. Immunol., 2004-07-01;173(1):21-4. 2004-07-01 [PMID: 15210754] (Mouse)
• Russell TD, Yan Q, Fan G, Khalifah AP, Bishop DK, Brody SL, Walter MJ IL-12 p40 homodimer-dependent macrophage chemotaxis and respiratory viral inflammation are mediated through IL-12 receptor beta 1. J. Immunol., 2003-12-15;171(12):6866-74. 2003-12-15 [PMID: 14662893] (Mouse)
• Koh YS, Yun JH, Seong SY, Choi MS, Kim IS Chemokine and cytokine production during Orientia tsutsugamushi infection in mice. Microb. Pathog., 2004-01-01;36(1):51-7. 2004-01-01 [PMID: 14643640] (Mouse)
• Huaux F, 107322, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D A profibrotic function of IL-12p40 in experimental pulmonary fibrosis. Interferon-gamma modulates articular chondrocyte and osteoblast metabolism through protein kinase R-independent and dependent mechanisms, 2002-09-01;169(5):2653-61. 2002-09-01 [PMID: 12193738] (Mouse)
• Hixon JA, 107284, Anver MR, Blazar BR, Panoskaltsis-Mortari A, Wiltrout RH, Murphy WJ Administration of either anti-CD40 or interleukin-12 following lethal total body irradiation induces acute lethal toxicity affecting the gut. Gene mutations in sporadic lymphangioleiomyomatosis and genotype-phenotype correlation analysis, 2002-01-01;8(6):316-25. 2002-01-01 [PMID: 12108917] (Mouse)
• Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls. Eur. J. Immunol., 2001-07-01;31(7):2104-15. 2001-07-01 [PMID: 11449364] (Mouse)
• Tkaczyk C, Villa I, Peronet R, David B, Chouaib S, Mecheri S In vitro and in vivo immunostimulatory potential of bone marrow-derived mast cells on B- and T-lymphocyte activation. J. Allergy Clin. Immunol., 2000-01-01;105(1):134-42. 2000-01-01 [PMID: 10629463] (Mouse)

Bioinformatics

• Gene Symbol: Il12b