Mouse E-Selectin/CD62E Quantikine ELISA Kit

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Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Mouse E-Selectin/CD62E Quantikine ELISA Kit Summary

Background
The Quantikine Mouse soluble E-Selectin (sE-Selectin) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sE-selectin levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sE-selectin and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sE-selectin showed linear curves that wer...e parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sE-selectin.
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Specificity
Natural and recombinant mouse E-Selectin
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Sele

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using MES00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse E-Selectin/CD62E Quantikine ELISA Kit

  • CD62 antigen-like family member E
  • CD62E antigen
  • CD62E
  • ELAM
  • ELAM1
  • ELAM-1
  • ELAM1E-selectin
  • endothelial adhesion molecule 1
  • Endothelial leukocyte adhesion molecule 1
  • ESEL
  • E-Selectin
  • LECAM2
  • leukocyte endothelial cell adhesion molecule 2
  • Leukocyte-endothelial cell adhesion molecule 2
  • SELE
  • selectin E

Background

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for E-Selectin/CD62E (MES00)(15)

We have publications tested in 1 confirmed species: Mouse.


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Mouse
(15)
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using MES00 Applications Species
S Miranda, P Billoir, L Damian, PA Thiebaut, D Schapman, M Le Besnera, F Jouen, L Galas, H Levesque, V Le Cam-Duc, R Joannides, V Richard, Y Benhamou Hydroxychloroquine reverses the prothrombotic state in a mouse model of antiphospholipid syndrome: Role of reduced inflammation and endothelial dysfunction PLoS ONE, 2019;14(3):e0212614. 2019 [PMID: 30870459] (Mouse) Mouse
Fabiola González-H Simvastatin attenuates endothelial activation through 15-epi-lipoxin A4 production in murine chronic Chagas cardiomyopathy Antimicrob. Agents Chemother, 2016;0(0):. 2016 [PMID: 27993857] (Mouse) Mouse
Shin HS, Xu F, Bagchi A, Herrup E, Wilhelmsen K Bacterial lipoprotein TLR2 agonists broadly modulate endothelial function and coagulation pathways in vitro and in vivo. J. Immunol., 2011;186(2):1119-30. 2011 [PMID: 21169547] (Mouse) Mouse
Deng Y, Edin ML, Theken KN, Schuck RN, Flake GP, Kannon MA, DeGraff LM, Lih FB, Foley J, Bradbury JA, Graves JP, Tomer KB, Falck JR, Zeldin DC, Lee CR Endothelial CYP epoxygenase overexpression and soluble epoxide hydrolase disruption attenuate acute vascular inflammatory responses in mice. FASEB J., 2011;25(2):703-13. 2011 [PMID: 21059750] (Mouse) Mouse
Sio SW, Moochhala S, Lu J Early protection from burn-induced acute lung injury by deletion of preprotachykinin-A gene. Am. J. Respir. Crit. Care Med., 2010;181(1):36-46. 2010 [PMID: 19797759] (Mouse) Mouse
Kahn P, Ramanujam M, Bethunaickan R, Huang W, Tao H, Madaio MP, Factor SM, Davidson A Prevention of murine antiphospholipid syndrome by BAFF blockade. Arthritis Rheum., 2008;58(9):2824-34. 2008 [PMID: 18759321] (Mouse) Mouse
Chiang LY, Sheppard DC, Gravelat FN, Patterson TF, Filler SG Aspergillus fumigatus stimulates leukocyte adhesion molecules and cytokine production by endothelial cells in vitro and during invasive pulmonary disease. Infect. Immun., 2008;76(8):3429-38. 2008 [PMID: 18490455] (Mouse) Mouse
Frei AC, Guo Y, Jones DW, Pritchard KA, Fagan KA, Hogg N, Wandersee NJ Vascular dysfunction in a murine model of severe hemolysis. Blood, 2008;112(2):398-405. 2008 [PMID: 18477769] (Mouse) Mouse
Westerterp M, Berbee JF, Pires NM, van Mierlo GJ, Kleemann R, Romijn JA, Havekes LM, Rensen PC Apolipoprotein C-I is crucially involved in lipopolysaccharide-induced atherosclerosis development in apolipoprotein E-knockout mice. Circulation, 2007;116(19):2173-81. 2007 [PMID: 17967778] (Mouse) Mouse
Zhang H, Zhi L, Moochhala SM, Moore PK, Bhatia M Endogenous hydrogen sulfide regulates leukocyte trafficking in cecal ligation and puncture-induced sepsis. J. Leukoc. Biol., 2007;82(4):894-905. 2007 [PMID: 17599903] (Mouse) Mouse
Show All 15 Publications.

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Product General Protocols

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FAQs for E-Selectin/CD62E (MES00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP MES00

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Gene Symbol Sele