Mouse E-Selectin/CD62E Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse E-Selectin/CD62E Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse soluble E-Selectin (sE-Selectin) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse sE-selectin levels in cell culture supernates, serum, and EDTA plasma. It contains NS0-expressed recombinant mouse sE-selectin and antibodies raised against the recombinant protein. Results obtained for naturally occurring mouse sE-selectin showed linear curves that wer...e parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural mouse sE-selectin.
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• Specificity: Natural and recombinant mouse E-Selectin
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Sele

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse E-Selectin/CD62E Quantikine ELISA Kit

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for E-Selectin/CD62E (MES00)(19)

Publications using MES00

• VP Kumar, S Biswas, GP Holmes-Ham, M Sheleg, S Stone, B Legesse, R Ofir, SP Ghosh Pre-Administration of PLX-R18 Cells Protects Mice from Radiation-Induced Hematopoietic Failure and Lethality Genes, 2022-09-28;13(10):. 2022-09-28 [PMID: 36292639] (Mouse)
• X Kassianide, V Allgar, IC Macdougall, PA Kalra, S Bhandari Analysis of oxidative stress, inflammation and endothelial function following intravenous iron in chronic kidney disease in the Iron and Heart Trial Scientific Reports, 2022-04-27;12(1):6853. 2022-04-27 [PMID: 35477731] (Human)
• S Thangaswam, CA Branch, K Ambadipudi, SA Acharya Quercetin Completely Ameliorates Hypoxia-Reoxygenation-Induced Pathophysiology Severity in NY1DD Transgenic Sickle Mice: Intrinsic Mild Steady State Pathophysiology of the Disease in NY1DD Is Also Reversed Biomolecules, 2021-10-06;11(10):. 2021-10-06 [PMID: 34680105] (Mouse)
• J Venugopal, J Wang, J Mawri, C Guo, D Eitzman Interleukin-1 receptor inhibition reduces stroke size in a murine model of sickle cell disease Haematologica, 2021-09-01;0(0):. 2021-09-01 [PMID: 32817286] (Mouse)
• S Miranda, P Billoir, L Damian, PA Thiebaut, D Schapman, M Le Besnera, F Jouen, L Galas, H Levesque, V Le Cam-Duc, R Joannides, V Richard, Y Benhamou Hydroxychloroquine reverses the prothrombotic state in a mouse model of antiphospholipid syndrome: Role of reduced inflammation and endothelial dysfunction PLoS ONE, 2019-03-14;14(3):e0212614. 2019-03-14 [PMID: 30870459] (Mouse)
• Fabiola González-H Simvastatin attenuates endothelial activation through 15-epi-lipoxin A4 production in murine chronic Chagas cardiomyopathy Antimicrob. Agents Chemother, 2017-02-23;0(0):. 2017-02-23 [PMID: 27993857] (Mouse)
• Shin HS, Xu F, Bagchi A, Herrup E, Wilhelmsen K Bacterial lipoprotein TLR2 agonists broadly modulate endothelial function and coagulation pathways in vitro and in vivo. J. Immunol., 2010-12-17;186(2):1119-30. 2010-12-17 [PMID: 21169547] (Mouse)
• Deng Y, Edin ML, Theken KN, Schuck RN, Flake GP, Kannon MA, DeGraff LM, Lih FB, Foley J, Bradbury JA, Graves JP, Tomer KB, Falck JR, Zeldin DC, Lee CR Endothelial CYP epoxygenase overexpression and soluble epoxide hydrolase disruption attenuate acute vascular inflammatory responses in mice. FASEB J., 2010-11-08;25(2):703-13. 2010-11-08 [PMID: 21059750] (Mouse)
• Sio SW, Moochhala S, Lu J Early protection from burn-induced acute lung injury by deletion of preprotachykinin-A gene. Am. J. Respir. Crit. Care Med., 2009-10-01;181(1):36-46. 2009-10-01 [PMID: 19797759] (Mouse)
• Kahn P, Ramanujam M, Bethunaickan R, Huang W, Tao H, Madaio MP, Factor SM, Davidson A Prevention of murine antiphospholipid syndrome by BAFF blockade. Arthritis Rheum., 2008-09-01;58(9):2824-34. 2008-09-01 [PMID: 18759321] (Mouse)
• Chiang LY, Sheppard DC, Gravelat FN, Patterson TF, Filler SG Aspergillus fumigatus stimulates leukocyte adhesion molecules and cytokine production by endothelial cells in vitro and during invasive pulmonary disease. Infect. Immun., 2008-05-19;76(8):3429-38. 2008-05-19 [PMID: 18490455] (Mouse)
• Frei AC, Guo Y, Jones DW, Pritchard KA, Fagan KA, Hogg N, Wandersee NJ Vascular dysfunction in a murine model of severe hemolysis. Blood, 2008-05-13;112(2):398-405. 2008-05-13 [PMID: 18477769] (Mouse)
• Westerterp M, Berbee JF, Pires NM, van Mierlo GJ, Kleemann R, Romijn JA, Havekes LM, Rensen PC Apolipoprotein C-I is crucially involved in lipopolysaccharide-induced atherosclerosis development in apolipoprotein E-knockout mice. Circulation, 2007-10-22;116(19):2173-81. 2007-10-22 [PMID: 17967778] (Mouse)
• Zhang H, Zhi L, Moochhala SM, Moore PK, Bhatia M Endogenous hydrogen sulfide regulates leukocyte trafficking in cecal ligation and puncture-induced sepsis. J. Leukoc. Biol., 2007-06-28;82(4):894-905. 2007-06-28 [PMID: 17599903] (Mouse)
• Nakamura M, Shimizu Y, Sato Y, Miyazaki Y, Satoh T, Mizuno M, Kato Y, Hosaka Y, Furusako S Toll-like receptor 4 signal transduction inhibitor, M62812, suppresses endothelial cell and leukocyte activation and prevents lethal septic shock in mice. Eur. J. Pharmacol., 2007-06-05;569(3):237-43. 2007-06-05 [PMID: 17588563] (Mouse)
• McCarter SD, Mei SH, Lai PF, Zhang QW, Parker CH, Suen RS, Hood RD, Zhao YD, Deng Y, Han RN, Dumont DJ, Stewart DJ Cell-based angiopoietin-1 gene therapy for acute lung injury. Am. J. Respir. Crit. Care Med., 2007-02-22;175(10):1014-26. 2007-02-22 [PMID: 17322110] (Mouse)
• Xu H, Ploplis VA, Castellino FJ A coagulation factor VII deficiency protects against acute inflammatory responses in mice. J. Pathol., 2006-12-01;210(4):488-96. 2006-12-01 [PMID: 17054310] (Mouse)
• Cruz-Topete D, Iwaki T, Ploplis VA, Castellino FJ Delayed inflammatory responses to endotoxin in fibrinogen-deficient mice. J. Pathol., 2006-11-01;210(3):325-33. 2006-11-01 [PMID: 16981239] (Mouse)
• Rein D, Schijlen E, Kooistra T, Herbers K, Verschuren L, Hall R, Sonnewald U, Bovy A, Kleemann R Transgenic flavonoid tomato intake reduces C-reactive protein in human C-reactive protein transgenic mice more than wild-type tomato. J. Nutr., 2006-09-01;136(9):2331-7. 2006-09-01 [PMID: 16920850] (Mouse)

FAQs for E-Selectin/CD62E (MES00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Sele