Mouse E-Selectin/CD62E DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse E-Selectin/CD62E DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Sele

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse E-Selectin/CD62E DuoSet ELISA, 15 Plate

• CD62 antigen-like family member E
• CD62E antigen
• CD62E
• ELAM
• ELAM1
• ELAM-1
• ELAM1E-selectin
• endothelial adhesion molecule 1
• Endothelial leukocyte adhesion molecule 1
• ESEL
• E-Selectin
• LECAM2
• leukocyte endothelial cell adhesion molecule 2
• Leukocyte-endothelial cell adhesion molecule 2
• SELE
• selectin E

Background

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.

Publications for E-Selectin/CD62E (DY575)(8)

Publications using DY575

• Vangrieken, P;Scheijen, JLJM;Schiffers, PMH;van de Waarenburg, MPH;Foulquier, S;Schalkwijk, CCG; Modelling the effects of elevated methylglyoxal levels on vascular and metabolic complications Scientific reports 2025-02-19 [PMID: 39972072] (Mouse)
• F Seidel, R Kleemann, W van Duyven, N van Trigt, N Keijzer, S van der Ko, C van Kooten, L Verschuren, A Menke, AJ Kiliaan, J Winter, TR Hughes, BP Morgan, F Baas, K Fluiter, MC Morrison Therapeutic Intervention with Anti-Complement Component 5 Antibody Does Not Reduce NASH but Does Attenuate Atherosclerosis and MIF Concentrations in Ldlr-/-.Leiden Mice International Journal of Molecular Sciences, 2022-09-14;23(18):. 2022-09-14 [PMID: 36142647] (Mouse)
• D Shah, P Das, S Acharya, B Agarwal, DJ Christense, SM Robertson, V Bhandari Small Immunomodulatory Molecules as Potential Therapeutics in Experimental Murine Models of Acute Lung Injury (ALI)/Acute Respiratory Distress Syndrome (ARDS) International Journal of Molecular Sciences, 2021-03-04;22(5):. 2021-03-04 [PMID: 33806560] (Mouse)
• KJ Kim, SH Kwon, JH Yun, HS Jeong, HR Kim, EH Lee, SK Ye, CH Cho STAT3 activation in endothelial cells is important for tumor metastasis via increased cell adhesion molecule expression Oncogene, 2017-05-22;0(0):. 2017-05-22 [PMID: 28534515] (Mouse)
• Shih M, Cherng J Reduction of adhesion molecule production and alteration of eNOS and endothelin-1 mRNA expression in endothelium by Euphorbia hirta L. through its beneficial beta-amyrin molecule. Molecules, 2014-07-18;19(7):10534-45. 2014-07-18 [PMID: 25045892] (Mouse)
• Hamers A, Uleman S, van Tiel C, Kruijswijk D, van Stalborch A, Huveneers S, de Vries C, van 't Veer C Limited role of nuclear receptor Nur77 in Escherichia coli-induced peritonitis. Infect Immun, 2013-10-28;82(1):253-64. 2013-10-28 [PMID: 24166953] (Mouse)
• Marut W, Kavian N, Servettaz A, Hua-Huy T, Nicco C, Chereau C, Weill B, Dinh-Xuan A, Batteux F Amelioration of systemic fibrosis in mice by angiotensin II receptor blockade. Arthritis Rheum, 2013-05-01;65(5):1367-77. 2013-05-01 [PMID: 23335130] (Mouse)
• Hegde A, Zhang H, Moochhala SM, Bhatia M Neurokinin-1 receptor antagonist treatment protects mice against lung injury in polymicrobial sepsis. J. Leukoc. Biol., 2007-06-12;82(3):678-85. 2007-06-12 [PMID: 17565047] (Mouse)

FAQs for E-Selectin/CD62E (DY575). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Sele