Mouse Dkk-1 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse Dkk-1 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse Dkk-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Dkk-1 in cell culture supernates, mouse serum, and plasma. It contains NS0-expressed recombinant mouse Dkk-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse Dkk-1. Results obtained using natural mouse Dkk-1 showed dose... response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse Dkk-1.
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• Specificity: Natural and recombinant mouse Dkk-1. This assay cross-reacts 94% with recombinant Rat Dkk-1 and 26% with recombinant Human Dkk-1.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Dkk1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Dkk-1 Quantikine ELISA Kit

• dickkopf (Xenopus laevis) homolog 1
• dickkopf homolog 1 (Xenopus laevis)
• dickkopf related protein-1
• Dickkopf-1
• dickkopf-related protein 1
• Dkk1
• Dkk-1
• hDkk-1
• SKdickkopf-1 like

Background

Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members. Soggy has not been shown to inhibit Wnt signaling, and its role in the pathway is unclear.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Dkk-1 (MKK100)(6)

Publications using MKK100

• YC Hsu, CC Chang, CC Hsieh, YT Huang, YH Shih, HC Chang, PJ Chang, CL Lin Dickkopf-1 Acts as a Profibrotic Mediator in Progressive Chronic Kidney Disease International Journal of Molecular Sciences, 2023-04-21;24(8):. 2023-04-21 [PMID: 37108841] (Rat)
• W Liang, P Han, EH Kim, J Mak, R Zhang, AG Torrente, JI Goldhaber, E Marbán, HC Cho Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells Stem Cells, 2019-12-30;0(0):. 2019-12-30 [PMID: 31648393] (Mouse)
• Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms Nat. Med, 2016-12-05;0(0):. 2016-12-05 [PMID: 27918563] (Mouse)
• Leukocyte Beta-Catenin Expression Is Disturbed in Systemic Lupus Erythematosus PLoS ONE, 2016-08-22;11(8):e0161682. 2016-08-22 [PMID: 27548498] (Mouse)
• Fowler J, Mundy G, Lwin S, Edwards C Bone marrow stromal cells create a permissive microenvironment for myeloma development: a new stromal role for Wnt inhibitor Dkk1. Cancer Res, 2012-02-28;72(9):2183-9. 2012-02-28 [PMID: 22374979] (Mouse)
• Genetos DC, Yellowley CE, Loots GG Prostaglandin E(2) Signals Through PTGER2 to Regulate Sclerostin Expression. PLoS ONE, 2011-03-16;6(3):e17772. 2011-03-16 [PMID: 21436889] (Mouse)

FAQs for Dkk-1 (MKK100). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Dkk1