Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) - BSA Free

Western Blot: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879] - Western Blot analysis of Mono- and polyubiquitinylated conjugates Antibody (HRP conjugated) detecting ubiquitin chains: Lane 1: MW Marker, Lane 2: K48-linked, Lane 3: K63-linked.

Immunocytochemistry/Immunofluorescence: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879] - Immunocytochemistry analysis of ubiquitinylated protein conjugates using FITC labeled Mono- and polyubiquitinylated conjugates Antibody. HeLa cells, pre-treated with proteasome inhibitor MG-132 at 5 uM for 12 hours prior to analysis with antibody at 1:250 dilution. Panel A: isotype control; Panel B: untreated; Panel C: proteasome inhibitor-treated HeLa cells. Left: 20X and Right: 63X amplified.

Flow Cytometry: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) [NBP3-06879] - Flow cytometry analysis of 106 Jurkat cells stained using Mono- and polyubiquitinylated conjugates Antibody (FITC conjugated), at a concentration of 10ug/ml, side-by-side comparison to another Mono- and polyubiquitinylated conjugates clone (FK2) (fluorescein labeled).

• Reactivity: All-NA
• Applications: WB, ELISA, Flow, ICC/IF
• Clone: UBCJ2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) - BSA Free Summary

• Immunogen: Poly-ubiquitinylated lysozyme.
• Specificity: Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) has been shown to detect K48 and K63-linked mono- and polyubiquitinylated proteins. Although not tested, in theory, this antibody should react to all mono- and polyubiquitinylated protein conjugates, regardless the linkage (K6, K11, K27, K29, K33, K48 or K63).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Western Blot 1:1000
• Application Notes: Suggested dilutions/conditions may not be appropriate for all applications. Optimal conditions must be determined individually for each application.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Mono- and polyubiquitinylated conjugates Antibody (UBCJ2) - BSA Free

• HEL-S-50
• polyubiquitin B
• polyubiquitin-B
• UBB
• UBC
• ubiquitin B
• ubiquitin C

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBC