MOG1 Antibody - BSA Free

Western Blot: MOG1 Antibody [NBP3-12671] - Lane A: HEK293 Whole Cell LysateLane B: MOLT4 Whole Cell LysateLane C: Daudi Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:20 kDaObserved band size:24 kDa

Immunohistochemistry-Paraffin: MOG1 Antibody [NBP3-12671] - Staining of human RANGRF in human testis with rabbit polyclonal antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: MOG1 Antibody [NBP3-12671] - Staining of human RANGRF in human kidney with rabbit polyclonal antibody at 1:500 dilution.

Immunoprecipitation: MOG1 Antibody [NBP3-12671] - Lane A:0.5 mg Daudi Whole Cell Lysate4 uL anti-RANGRF rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-RANGRF rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size :20 kDa

Immunocytochemistry/Immunofluorescence: MOG1 Antibody [NBP3-12671] - Staining of RANGRF in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12671 antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MOG1 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human MOG1 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RANGRF
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MOG1 Antibody - BSA Free

• HSPC165
• HSPC236
• MOG1 homolog
• MOG1DKFZp686F02139
• RAN guanine nucleotide release factor
• Ran-binding protein MOG1
• RanGNRF
• RANGNRFMGC110973

Background

MOG1 may regulate the intracellular trafficking of RAN. In cardiac cells seems to regulate the cell surface localization of SCN5A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RANGRF