Moesin Antibody

Western Blot: Moesin Antibody [NBP2-19376] - A. 30 ug GL261 whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug Raw264.7 whole cell lysate/extract F. 30 ug C2C12 whole cell lysate/extract 7.5 % SDS-PAGE Moesin antibody [C2C3], C-term dilution: 1:1000

Immunocytochemistry/Immunofluorescence: Moesin Antibody [NBP2-19376] - Confocal immunofluorescence analysis of methanol-fixed A431, using Moesin antibody (green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:500.

Immunohistochemistry-Paraffin: Moesin Antibody [NBP2-19376] - Paraffin-embedded rat spleen. Moesin antibody [C2C3], C-term diluted at 1:500.

Immunoprecipitation: Moesin Antibody [NBP2-19376] - Immunoprecipitation of Moesin protein from A431 whole cell extracts using 5 ug of Moesin antibody [C2C3], C-term. Western blot analysis was performed using Moesin antibody [C2C3], C-term. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

Western Blot: Moesin Antibody [NBP2-19376] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Moesin antibody [C2C3], C-term (NBP2-19376) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Moesin antibody [C2C3], C-term detects MSN protein by Western blot analysis.A. 30 ug GL261 whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug Raw264.7 whole cell lysate/extract F. 30 ug C2C12 whole cell lysate/extract 7.5 % SDS-PAGEMoesin antibody [C2C3], C-term (NBP2-19376) dilution: 1:1000

• Reactivity: Hu, Mu, Rt, Po, Bv, RM
• Applications: WB, ICC/IF, IHC, IP, KO
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Moesin Antibody Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit Moesin Antibody (NBP2-19376) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Moesin. The exact sequence is proprietary.
• Localization: Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Cytoplasm; cytoskeleton (By similarity); Apical cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Cell projection; microvillus membrane; Periphe
• Predicted Species: Porcine (98%), Bovine (99%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MSN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Moesin Antibody

• Membrane-organizing extension spike protein
• moesin

Background

Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

FAQs for Moesin Antibody (NBP2-19376). (Showing 1 - 1 of 1 FAQ).

1. I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.
   • The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining <a href="http://en.wikipedia.org/wiki/RNA_interference" target="_blank">RNA interference</a>. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

Bioinformatics

• Gene Symbol: MSN
• Uniprot:
  • P26038(Human)