Moesin Antibody

Images

 
Western Blot: Moesin Antibody [NBP2-19376] - A. 30 ug GL261 whole cell lysate/extract B. 30 ug C8D30 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug ...read more
Immunocytochemistry/ Immunofluorescence: Moesin Antibody [NBP2-19376] - Confocal immunofluorescence analysis of methanol-fixed A431, using Moesin antibody (green) at 1:500 dilution. Alpha-tubulin filaments are labeled ...read more
Immunohistochemistry-Paraffin: Moesin Antibody [NBP2-19376] - Paraffin-embedded rat spleen. Moesin antibody [C2C3], C-term diluted at 1:500.
Western Blot: Moesin Antibody [NBP2-19376] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunoprecipitation: Moesin Antibody [NBP2-19376] - Immunoprecipitation of Moesin protein from A431 whole cell extracts using 5 ug of Moesin antibody [C2C3], C-term. Western blot analysis was performed using Moesin ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.21 mg/ml

Order Details

Moesin Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human Moesin. The exact sequence is proprietary.
Localization
Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Cytoplasm; cytoskeleton (By similarity); Apical cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity); Cell projection; microvillus membrane; Periphe
Predicted Species
Porcine (98%), Bovine (99%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MSN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.21 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Moesin Antibody

  • Membrane-organizing extension spike protein
  • moesin

Background

Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Moesin Antibody (NBP2-19376). (Showing 1 - 1 of 1 FAQ).

  1. I am looking to use shRNA to inhibit Moesin expression. I have had people advise me that my initial MOI should be low as 'less is more' and 'a little goes a long way' in terms of siRNA. I was wondering if you could elaborate on this for me and explain why my initial MOI should be low.
    • The reason for a low MOI is most likely because RNAi is a very strong and efficient technique. Wikipedia does a good job of explaining <a href="http://en.wikipedia.org/wiki/RNA_interference" target="_blank">RNA interference</a>. However, I would imagine that in a cell, there will be at most 1-2 copies of the gene mRNA present at any given time, unless you're dealing with a highly expressed protein such as Actin, where I would imagine silencing Actin would be lethal to the cell. I can imagine a few reasons to not use too much siRNA. First, it is expensive, so you don't want to waste it. Second, using too much would cause there to be a lot of non-translatable RNA present in the cell, which could trigger an immune response, as the presence of uncapped RNAs can indicate presence of a virus and one of the TLRs may respond to this.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MSN
Uniprot

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