MMR/CD206/Mannose Receptor Antibody (685645)

Western blot shows lysates of human immature dendritic cells. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMR/CD206 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMR/CD206 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25341) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in sinusoids. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of human immature dendritic cells, loaded at 0.2 mg/mL. A specific band was detected for MMR/CD206 at approximately 228 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human MMR/CD206 Monoclonal Antibody (Catalog # MAB25341). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clone: 685645
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MMR/CD206/Mannose Receptor Antibody (685645) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMR/CD206
  Leu19-Lys1383 (Thr399Ala) & (Leu407Phe),
  Accession # P22897
• Specificity: Detects human MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) MMR or recombinant human Mrc2 is observed. In Western blots, approximately 25% cross-reactivity with rmMMR is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRC1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMR/CD206/Mannose Receptor Antibody (685645)

• CD206
• CLEC13D
• CLEC13Dmacrophage mannose receptor 1
• C-type lectin domain family 13 member D
• mannose receptor, C type 1
• MMR
• MMRCD206 antigen
• MRC1

Background

The human Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 190 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The human MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1371 aa extracellular region, a 21 aa transmembrane segment and a 46 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3, 4). Human and mouse MMR extracellular regions share 82% aa identity. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (5). Several of the CRDs participate in the Ca²⁺-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (6). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor is recycled to the cell surface (3, 7). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
4. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
5. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
6. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
7. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMR/CD206/Mannose Receptor Antibody (MAB25341)(10)

Publications using MAB25341

• Abu El-Asrar AM, De Hertogh G, Allegaert E et al. Macrophage-Myofibroblast Transition Contributes to Myofibroblast Formation in Proliferative Vitreoretinal Disorders International journal of molecular sciences 8/31/2023 [PMID: 37686317]
• Jesus Maria Gómez-Salinero, Franco Izzo, Yang Lin, Sean Houghton, Tomer Itkin, Fuqiang Geng et al. Specification of fetal liver endothelial progenitors to functional zonated adult sinusoids requires c-Maf induction Cell Stem Cell 4/1/2022 [PMID: 35364013]
• Ayumi Hashimoto, Debashis Sarker, Vikash Reebye, Sheba Jarvis, Mikael H. Sodergren, Andrew Kossenkov et al. Upregulation of C/EBP alpha Inhibits Suppressive Activity of Myeloid Cells and Potentiates Antitumor Response in Mice and Patients with Cancer Clinical Cancer Research 11/1/2021 [PMID: 34407972]
• Choi YW, Kim YH, Oh SY et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer Advanced Science 1/4/2021 [PMID: 33643790]
• T Kazuo, Y Suzuki, K Yoshimura, H Yasui, M Karayama, H Hozumi, K Furuhashi, N Enomoto, T Fujisawa, Y Nakamura, N Inui, K Yokomura, T Suda Macrophage Mannose Receptor CD206 Predicts Prognosis in Community-acquired Pneumonia Sci Rep, 2019-12-10;9(1):18750. 2019-12-10 [PMID: 31822747] (IHC-P, Human)
• Min Zhang, Kojiro Nakamura, Shoichi Kageyama, Akeem O. Lawal, Ke Wei Gong, May Bhetraratana et al. Myeloid HO-1 modulates macrophage polarization and protects against ischemia-reperfusion injury JCI Insight 10/4/2018 [PMID: 30282830]
• NS Harasymowi, ND Clement, A Azfer, R Burnett, DM Salter, AH Simpson Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and III Obesity in Patients with OA Arthritis & rheumatology (Hoboken, N.J.), 2017-06-10;0(0):. 2017-06-10 [PMID: 28320058] (IHC, Human)
• Wang Xin, Yao Bing, Wang Yinqiu et al. Macrophage Cyclooxygenase-2 Protects Against Development of Diabetic Nephropathy. Diabetes 1/1/2017 [PMID: 27815317]
• Zhang Ming-Zhi, Wang Xin, Wang Yinqiu et al. IL-4/IL-13-mediated polarization of renal macrophages/dendritic cells to an M2a phenotype is essential for recovery from acute kidney injury. Kidney International 1/1/2017 [PMID: 27745702]
• Guido Carpino Macrophage Activation in Pediatric Nonalcoholic Fatty Liver Disease (NAFLD) Correlates with Hepatic Progenitor Cell Response via Wnt3a Pathway PLoS ONE, 2016-06-16;11(6):e0157246. 2016-06-16 [PMID: 27310371] (IHC-P, Human)

Bioinformatics

• Gene Symbol: MRC1
• Uniprot:
  • P22897()