MMP-9 Antibody (S51-82) - BSA Free

Western Blot: MMP-9 Antibody (S51-82) [NBP2-59699] - Western Blot analysis of Rat Brain showing detection of ~92 kDa and ~82 kDa (pro and active) MMP-9 protein using Mouse Anti-MMP-9 Monoclonal Antibody, Clone S51-82 (NBP2-59699). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-MMP-9 Monoclonal Antibody (NBP2-59699) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~92 kDa and ~82 kDa (pro and active).

Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-MMP-9 Monoclonal Antibody, Clone S51-82 (NBP2-59699). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-MMP-9 Monoclonal Antibody (NBP2-59699) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) MMP-9 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699] - Analysis using Mouse Anti-MMP9 Monoclonal Antibody, Clone S51-82 . Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-MMP9 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) MMP9 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: MMP-9 Antibody (S51-82) [NBP2-59699] - Identification of MET-like structures within human fetal membrane tissues infected with GBS ex vivo. Fetal membrane tissues were excised from healthy, term placental tissues from women undergoing routine cesarean section. (A and B) Fetal membrane tissues were then infected with GBS on the choriodecidual surface for 48 h prior to fixation and confocal microscopy. Fixed and paraffin-embedded fetal membranes were stained with conjugated primary antibodies against CD163, histone H3, and MMP-9. CD163-positive cells within the membrane tissue are seen extruding contents that stain positive for histones and MMP-9 consistent with METs (white arrowheads). Bars represent 20 um. Image collected and cropped by CiteAb from the following publication (//mbio.asm.org/lookup/doi/10.1128/mBio.02084-18) licensed under a CC-BY license.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: S51-82
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MMP-9 Antibody (S51-82) - BSA Free Summary

• Immunogen: Fusion protein amino acids 1-708 (full length) of rat MMP9
• Localization: Extracellular Matrix
• Specificity: Detecs ~92kDa and ~82kDa (pro and active forms).
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: MMP9
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of this antibody was sufficient for detection of MMP9 in 20 ug of COS-1 cells (lysate) transfected with human MMP9 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for MMP-9 Antibody (S51-82) - BSA Free

• 92 kDa gelatinase
• 92 kDa type IV collagenase
• CLG4B
• EC 3.4.24
• EC 3.4.24.35
• Gelatinase B
• GELB
• macrophage gelatinase
• MANDP2
• matrix metallopeptidase 9
• matrix metalloproteinase 9
• matrix metalloproteinase-9
• MMP9
• MMP-9
• type V collagenase

Background

MMP9, otherwise known as matrix metalloproteinase 9, is involved in the breakdown of extracellular matrix in normal physiological processes such as embryonic development, reproduction and tissue remodeling, as well as in disease processes like arthritis and metastasis (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-9 Antibody (NBP2-59699)(1)

Publication using NBP2-59699

• Doster R, Sutton J, Rogers L et al. Streptococcus agalactiae induces placental macrophages to release extracellular traps loaded with tissue remodeling enzymes via an oxidative-burst-dependent mechanism. bioRxiv. 2018-10-11 [PMID: 30459195] (ICC/IF, Human)

FAQs for MMP-9 Antibody (NBP2-59699). (Showing 1 - 1 of 1 FAQ).

1.  I’m looking for a pair of antibodies to MMP-9 that can be used in a sandwich assay. Do you carry any? 
   • We have 10 primary antibodies for MMP-9 that have been tested in ELISA, seen here.It looks like 2 have been tested for capture (please note the tested species for each of these), seen here.6 have been tested for detection, seen here.

Bioinformatics

• Gene Symbol: MMP9