MMP-7 Antibody [Unconjugated]

Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMP-7 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of normal stem cells (negative control) and Crohn's stem cells, loaded at 0.2 mg/mL. A specific band was detected for MMP‑7 at approximately 10 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

The cleavage ofMca‑PLGL‑Dpa‑AR‑NH2 (10 μM, Catalog # ES001) by Recombinant Human MMP‑7 (0.2 µg/mL, Catalog # 907-MP) is measured after preincubation with increasing concentrations of Goat Anti-Human MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907). The ND50 is typically 2 µg/mL.

Formalin-fixed paraffin-embedded tissue sections of human pancreas were probed for MMP7 mRNA (ACD RNAScope Probe, catalog #488408; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human MMP7 polyclonal antibody (R&D Systems catalog # AF907) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to exocrine glands.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, IP, B/N, Dual ISH-IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MMP-7 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MMP‑7
  Leu18-Lys267
  Accession # P09237
• Specificity: Detects human MMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MMP-7 is observed, and less than 1% cross-reactivity with recombinant human (rh) MMP‑8 and rhMMP-12 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MMP7
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Neutralization 2 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-7 Antibody [Unconjugated]

• EC 3.4.24
• EC 3.4.24.23
• Matrilysin
• matrin
• matrix metallopeptidase 7 (matrilysin, uterine)
• matrix metalloproteinase 7 (matrilysin, uterine)
• Matrix metalloproteinase-7
• MMP7
• MMP-7
• MPSL1
• Pump-1 protease
• PUMP1
• PUMP-1
• uterine matrilysin
• Uterine metalloproteinase

Background

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-7 (matrilysin) is expressed in epithelial cells of normal and diseased tissues, and is capable of digesting a large series of proteins of the extracellular matrix including collagen IV and X, gelatin, casein, laminin, aggrecan, entactin, elastin and versican. MMP-7 is implicated in the activation of other proteinases such as plasminogen, MMP-1, MMP-2, and MMP-9. In addition to its roles in connective tissue remodeling and cancer, MMP-7 also regulates intestinal
alpha ‑defensin activation in innate host defense, releases tumor necrosis factor‑ alpha in a model of herniated disc resorption, and cleaves FasL to generate a soluble form in a model of prostate involution. Structurally, MMP-7 is the smallest of the MMPs and consists of two domains: a pro-domain that is cleaved upon activation and a catalytic domain containing the zinc-binding site.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMP-7 Antibody (AF907)(8)

Publications using AF907

• Ramamoorthy, R;Hussain, H;Ravelo, N;Sriramajayam, K;Di Gregorio, DM;Paulrasu, K;Chen, P;Young, K;Masciarella, AD;Jayakumar, AR;Paidas, MJ; Kidney Damage in Long COVID: Studies in Experimental Mice Biology 2023-07-30 [PMID: 37626956] (Western Blot, IHC, Mouse)
• A Abbes, Y Zayani, W Zidi, MB Hammami, A Mebazaa, D El Euch, A Ben Ammar, H Sanhaji, MV El May, M Mokni, M Feki, M Allal-Elas Matrix metalloproteinase-7 could be a predictor for acute inflammation in psoriatic patients Cytokine, 2020-07-11;134(0):155195. 2020-07-11 [PMID: 32663776] (IHC, Human)
• Satvik Hadigal, Raghuram Koganti, Tejabhiram Yadavalli, Alex Agelidis, Rahul Suryawanshi, Deepak Shukla Heparanase-Regulated Syndecan-1 Shedding Facilitates Herpes Simplex Virus 1 Egress Journal of Virology 2/28/2020 [PMID: 31827001]
• Z Peng, J Chen, CB Drachenber, JP Raufman, G Xie Farnesoid X receptor represses matrix metalloproteinase 7 expression, revealing this regulatory axis as a promising therapeutic target in colon cancer J. Biol. Chem., 2019-04-09;0(0):. 2019-04-09 [PMID: 30967475] (Western Blot, Human)
• Yingqiu Xie, Wenfu Lu, Shenji Liu, Qing Yang, J. Shawn Goodwin, Sandeep Anantha Sathyanarayana et al. MMP7 interacts with ARF in nucleus to potentiate tumor microenvironments for prostate cancer progression in vivo Oncotarget 7/26/2016 [PMID: 27356744]
• Steven T. Sizemore, Gina M. Sizemore, Christine N. Booth, Cheryl L. Thompson, Paula Silverman, Gurkan Bebek et al. Hypomethylation of the MMP7 promoter and increased expression of MMP7 distinguishes the basal-like breast cancer subtype from other triple-negative tumors Breast Cancer Research and Treatment 7/1/2014 [PMID: 24847890]
• Zhuo , Jingli, Tan , Ee Hong, Yan , Benedict, Tochhawng , Lalchhan, Jayapal , Manikand, Koh , Shiuan, Tay , Hwee Kee, Maciver , Sutherla, Hooi , Shing Ch, Salto-Tellez , Manuel, Kumar , Alan Pre, Goh , Yaw Chon, Lim , Yaw Chyn, Yap , Celestia Gelsolin induces colorectal tumor cell invasion via modulation of the urokinase-type plasminogen activator cascade. PLoS ONE, 2012-08-21;7(8):e43594. 2012-08-21 [PMID: 22927998] (Neutralization, Human)
• Hawinkels LJ, Verspaget HW, van den Berg M, Hanemaaijer R, Sier CF Determination of matrilysin activity in gastrointestinal neoplasia. Eur. J. Clin. Invest., 2007-07-01;37(7):598-9. 2007-07-01 [PMID: 17576214] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: MMP7
• Uniprot:
  • P09237()