MMP-2 Antibody (36006) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 36006
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

MMP-2 Antibody (36006) [Alexa Fluor® 488] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human MMP-2
• Specificity: Detects pro/active forms of human MMP-2 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) MMP‑9, rhMMP-1, or rhMMP-3 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMP-2 Antibody (36006) [Alexa Fluor® 488]

• 72 kDa gelatinase
• CLG4
• CLG4A72 kDa type IV collagenase
• collagenase type IV-A
• EC 3.4.24
• EC 3.4.24.24
• Gelatinase A
• matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)
• matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IVcollagenase)
• Matrix metalloproteinase-2
• matrix metalloproteinase-II
• MMP2
• MMP-2
• MMP-II
• MONA
• neutrophil gelatinase
• TBE-1matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase)

Background

Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-2 (gelatinase A), a type IV collagenase, can degrade a broad range of substrates including type IV, V, VII and X collagens as well as elastin and fibronectin. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-2 has been shown to be associated with many connective tissue cells as well as neutrophils, macrophages and monocytes. Structurally, MMP-2 may be divided into several distinct domains: a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a fibronectin-like domain thought to play a role in substrate targeting;and a carboxyl terminal (hemopexin-like) domain containing 2 N-linked glycosylation sites.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.