MLX Overexpression Lysate (Native)

Images

 
Western Blot: MLX Overexpression Lysate (Adult Normal) [NBP2-10013] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MLX.

Product Details

Summary
Applications WB

Order Details

MLX Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_733752. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens MLX, mRNA.
Gene
MLX

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
33.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MLX

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MLX Overexpression Lysate (Native)

  • BHLHD13
  • bHLHd13Max-like bHLHZip protein
  • BigMax alpha
  • BigMax protein
  • Class D basic helix-loop-helix protein 13
  • MAD7
  • MAX-like protein X
  • MLX
  • MXD7
  • TCFL4
  • TCFL4Protein BigMax
  • transcription factor-like 4
  • Transcription factor-like protein 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Blogs on MLX.

ChREBP, a glucose sensitive transcription factor with role in glucose-lipids homeostasis and cancer
ChREBP (carbohydrate response element-binding protein) is a glucose responsive basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that binds MLX and then carbohydrate response element /ChoRE for the induction of genes involved in...  Read full blog post.

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Bioinformatics

Gene Symbol MLX
Entrez
Uniprot

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