MLK4 Antibody - BSA Free

Western Blot: MLK4 Antibody [NBP1-90950] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Immunocytochemistry/Immunofluorescence: MLK4 Antibody [NBP1-90950] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.

Immunohistochemistry-Paraffin: MLK4 Antibody [NBP1-90950] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp

Staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.

Staining of human cell line A-431 shows localization to plasma membrane & cytosol.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MLK4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MLK4 Antibody - BSA Free (NBP1-90950) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GNPTPTGATIISATGASALPLCPSPAPHSHLPREVSPKKHSTVHIVPQRRPASLRSRSDLPQAYPQTAVSQLAQTACVVGRPGPHPTQFLAAKERTKSHVPSLLDADVEGQSRDYTVPLCRMRSKTSRPSIYELEKE
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAP3K21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MLK4 Protein (NBP1-90950PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MLK4 Antibody - BSA Free

• dJ862P8.3
• EC 2.7.11
• EC 2.7.11.25
• mitogen-activated protein kinase kinase kinase MLK4
• mixed lineage kinase 4
• MLK4
• RP5-862P8.2

Background

Activates the JUN N-terminal pathway

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP3K21