MLK3 Antibody (S07-2C3)

Western Blot: MLK3 Antibody (S07-2C3) [NBP3-19695] - Western blot detection of MLK3 in HepG2, A431 lysates using MLK3 antibody. Predicted band size: 93kDa. Observed band size: 93kDa.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: S07-2C3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

MLK3 Antibody (S07-2C3) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human MLK3 (Uniprot # Q16584)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAP3K11
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000
• Theoretical MW: 93 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for MLK3 Antibody (S07-2C3)

• EC 2.7.11
• EC 2.7.11.25
• MAP3K11
• MEKK11
• mitogen-activated protein kinase kinase kinase 11
• Mixed lineage kinase 3
• MLK3
• MLK3MLK-3
• PTK1
• PTK1protein-tyrosine kinase PTK1
• RHOE
• SH3 domain-containing proline-rich kinase
• SPRK
• SPRKMGC17114
• Src-homology 3 domain-containing proline-rich kinase

Background

Mixed lineage kinase-3 (MLK-3) is a mitogen-activated kinase kinase kinase that mediates stress-activating protein kinase (SAPK)/c-Jun NH2-terminal kinase activation. MLK-3 and other MLK family kinases are characterized by the presence of multiple protein-protein interaction domains including a tandem leucine/isoleucine zipper (LZs) motif. Leucine zippers are known to mediate protein dimerization raising the possibility that the tandem leucine/isoleucine zippers may function as a dimerization motif of MLK-3 (1). MLK-3 has several interesting structural features including an SH3 domain in the absence of an SH2 domain, a region containing two leucine zippers with an adjacent carboxy-terminal basic region, and a proline rich region. (2). HPK1 has been found to phosphorylate MLK-3 activation loop in vitro, and Ser281 was found to be the major phosphorylation site, indicating that HPK1 also activates MLK-3 via phosphorylation of the kinase activation loop (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP3K11