![Immunocytochemistry/Immunofluorescence: MKLP1 Antibody [NBP2-17299] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MKLP1 protein stained by MKLP1 antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200.](http://images.novusbio.com/fullsize/MKLP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17299-img0004.jpg "Immunocytochemistry/Immunofluorescence: MKLP1 Antibody [NBP2-17299] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MKLP1 protein stained by MKLP1 antibody [N1N2], N-term diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200.")
| Reactivity | Hu, Mu, Rt, BvSpecies Glossary |
| Applications | WB, ICC/IF |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human MKLP1. The exact sequence is proprietary. |
| Localization | Nucleus |
| Predicted Species | Mouse (91%), Rat (90%), Bovine (93%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | KIF23 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 110 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for MKLP1 Antibody (NBP2-17299)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: MKLP1 Antibody [NBP2-17299] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MKLP1 protein stained by MKLP1 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/MKLP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-17299-img0003.jpg "Immunocytochemistry/Immunofluorescence: MKLP1 Antibody [NBP2-17299] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MKLP1 protein stained by MKLP1 antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.")
![Western Blot: MKLP1 Antibody [NBP2-17299] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MKLP1 antibody [N1N2], N-term (NBP2-17299) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17299_rabbit-polyclonal-mklp1-antibody-91020242012200.jpg "Western Blot: MKLP1 Antibody [NBP2-17299] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MKLP1 antibody [N1N2], N-term (NBP2-17299) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: MKLP1 Antibody [NBP2-17299] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MKLP1 antibody [N1N2], N-term (NBP2-17299) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-17299_rabbit-polyclonal-mklp1-antibody-91020242001044.jpg "Western Blot: MKLP1 Antibody [NBP2-17299] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MKLP1 antibody [N1N2], N-term (NBP2-17299) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")