MKLP1 Antibody (9V4Y8)

Western Blot: MKLP1 Antibody (9V4Y8) [NBP3-16507] - Western blot analysis of extracts of various cell lines, using MKLP1 Rabbit mAb (NBP3-16507) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunoprecipitation: MKLP1 Antibody (9V4Y8) [NBP3-16507] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug MKLP1 antibody . Western blot was performed from the immunoprecipitate using MKLP1 antibody at a dilution of 1:1000.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, IP
• Clone: 9V4Y8
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MKLP1 Antibody (9V4Y8) Summary

• Description: Novus Biologicals Rabbit MKLP1 Antibody (9V4Y8) (NBP3-16507) is a recombinant monoclonal antibody validated for use in WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 801-960 of human MKLP1 (Q02241). NAPPIRLRHRRSRSAGDRWVDHKPASNMQTETVMQPHVPHAITVSVANEKALAKCEKYMLTHQELASDGEIETKLIKGDIYKTRGGGQSVQFTDIETLKQESPNGSRKRRSSTVAPAQPDGAESEWTDVETRCSVAVEMRAGSQLGPGYQHHAQPKRKKP
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KIF23
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoprecipitation 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MKLP1 Antibody (9V4Y8)

• kinesin family member 23
• kinesin-like 5 (mitotic kinesin-like protein 1)
• Kinesin-like protein 5
• kinesin-like protein KIF23
• KNSL5CHO1
• mitotic kinesin-like 1
• MKLP-1
• MKLP1Mitotic kinesin-like protein 1

Background

Using the CHO1 monoclonal antibody, Sellitto and Kuriyama (1988) identified a spindle antigen that was shown by Nislow et al. (1990) to be required for mitotic progression. By using the CHO1 antibody to screen a HeLa cell cDNA library, Nislow et al. (1992) isolated a KNSL5 cDNA, which they called MKLP1 (mitotic kinesin-like protein-1), that encodes this antigen. The N-terminal region of the deduced 961-amino acid protein contains a 350-residue domain that shares 30 to 45% identity with the motor domains of other members of the kinesin superfamily. The C-terminal region shows little identity with other kinesins. A central region contains heptad repeats of hydrophobic residues that may assemble into a coiled-coil structure similar to kinesin and myosin heavy chain proteins. The protein also contains a putative nuclear localization signal and 2 consensus phosphorylation sites characteristic of nuclear proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KIF23