MKL2 Antibody (CL1546) - BSA Free

Western Blot: MKL2 Antibody (CL1546) [NBP2-52970] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4

Immunocytochemistry/Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970] - Staining of A549 cells using the Anti-MKL2 monoclonal antibody, showing specific staining in the nucleus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunocytochemistry/Immunofluorescence: MKL2 Antibody (CL1546) [NBP2-52970] - Staining of A549 cells using the Anti-MKL2 monoclonal antibody, showing specific staining in nuclear speckles and the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: CL1546
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

MKL2 Antibody (CL1546) - BSA Free Summary

• Description: Novus Biologicals Mouse MKL2 Antibody (CL1546) - BSA Free (NBP2-52970) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q9ULH7, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRTFB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  MKL2 Recombinant Protein Antigen (NBP2-52970PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for MKL2 Antibody (CL1546) - BSA Free

• DKFZp686J1745
• FLJ31823
• FLJ45623
• KIAA1243
• megakaryoblastic leukemia 2
• MKL/myocardin-like 2
• MKL/myocardin-like protein 2
• MRTFB
• MRTF-B
• myocardin-related transcription factor B
• NPD001

Background

MKL2 acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRTFB