MKK7/MEK7 Antibody (324302) [DyLight 755]

• Reactivity: Hu
• Applications: IHC
• Clone: 324302
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 755

MKK7/MEK7 Antibody (324302) [DyLight 755] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: E. coli-derived recombinant human MKK7
  Met1-Arg419
  Accession # O14733
• Specificity: Detects rhMKK7 in direct ELISAs. This antibody does not cross-react with rhMKK4.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAP2K7
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product MAB3579. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for MKK7/MEK7 Antibody (324302) [DyLight 755]

• c-Jun N-terminal kinase kinase 2
• dual specificity mitogen-activated protein kinase kinase 7
• EC 2.7.12.2
• JNK kinase 2
• JNK-activating kinase 2
• JNKK 2
• JNKK2
• MAP kinase kinase 7
• MAP2K7
• MAPK/ERK kinase 7
• MAPKK7
• MEK 7
• MEK7
• mitogen-activated protein kinase kinase 7
• MKE7
• MKK7
• MKK7MAPKK 7
• PRKMK7

Background

c-Jun NH2-terminal protein kinase (JNK), a distant member of the mitogen-activated protein (MAP) kinase family, regulates gene expression in response to various extracellular stimuli. MKK7, a novel member of the MAP kinase kinase family, was phosphorylated and activated by MEKK1, a MAP kinase kinase kinase in the JNK signaling cascade. MKK7 activity was also stimulated by constitutively active forms of Rac and Cdc42Hs, members of the Rho small GTP-binding protein family (1). endogenous MKK7 was activated by treatment with the growth factor interleukin-3 (but not interleukin-4), or by ligation of CD40, the B-cell antigen receptor, or the receptor for the Fc fragment of immunoglobulin. MKK7 was also activated when cells were exposed to heat, UV irradiation, anisomycin, hyperosmolarity or the pro-inflammatory cytokine tumor necrosis factor-alpha, suggesting that MKK7 is involved in many physiological pathways (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K7