MKK4/MEK4 Antibody (5H4)

Western Blot: MKK4/MEK4 Antibody (5H4) [NBP2-37275] - Western blot analysis using MAP2K4 mouse mAb against HepG2 (1), K562 (2), and HEK293 (3) cell lysate.

Immunohistochemistry: MKK4/MEK4 Antibody (5H4) [NBP2-37275] - Immunohistochemical analysis of paraffin-embedded muscle tissues using MAP2K4 mouse mAb with DAB staining.

Flow Cytometry: MKK4/MEK4 Antibody (5H4) [NBP2-37275] - Flow cytometric analysis of K562 cells using MAP2K4 mouse mAb (green) and negative control (purple).

ELISA: MKK4/MEK4 Antibody (5H4) [NBP2-37275] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

MKK4/MEK4 Antibody (5H4) Summary

• Immunogen: Purified recombinant fragment of human MAP2K4 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MAP2K4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for MKK4/MEK4 Antibody (5H4)

• c-Jun N-terminal kinase kinase 1
• dual specificity mitogen-activated protein kinase kinase 4
• JNK activating kinase 1
• JNK-activated kinase 1
• JNK-activating kinase 1
• JNKK
• JNKK1
• JNKK1MAPK/ERK kinase 4
• MAP kinase kinase 4
• MAP2K4
• MAPKK4
• MEK4
• MEK4MEK 4
• mitogen-activated protein kinase kinase 4
• MKK4
• MKK4SAPK/ERK kinase 1
• PRKMK4
• PRKMK4MAPKK 4
• SEK1
• SERK1
• SERK1EC 2.7.12.2

Background

MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylates and activates p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K4