Mitofusin 1 Antibody (JF0954)

Western Blot: Mitofusin 1 Antibody (JF0954) [NBP2-66952] - Analysis of Mitofusin 1 on K562 cells lysates using anti-Mitofusin 1 antibody at 1/1,000 dilution.

Flow Cytometry: Mitofusin 1 Antibody (JF0954) [NBP2-66952] - Analysis of 293 cells with Mitofusin 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Product Discontinued

Mitofusin 1 Antibody (JF0954) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human Mitofusin 1 aa 491-620 / 741. (SwissProt: Q8IWA4 Human)
• Localization: Mitochondrion outer membrane, Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MFN1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Mitofusin 1 Antibody (JF0954)

• DKFZp762F247
• EC 3.6.5
• EC 3.6.5.-
• FLJ20693
• Fzo homolog
• hfzo1
• hfzo2
• MFN1
• MGC41806
• mitochondrial transmembrane GTPase Fzo-1
• mitochondrial transmembrane GTPase FZO-2
• Mitofusin 1
• mitofusin-1
• putative transmembrane GTPase
• Transmembrane GTPase MFN1

Background

MFN1 (Mitofusin-1) is a nuclear encoded mitochondrial membrane protein that mediates mitochondrial fusion and morphology in mammalian cells. MFN1 and MFN2 are homologs of the Drosophila protein Fuzzy Onion (Fzo) that interact with each other to facilitate mitochondrial targeting. MFN1 is ubiquitously expressed,with slightly higher leves detectedl in kidney and heart. MFN2 may be overexpressed in some tumors, such as lung cancers.

Mitochondrial fusion occurs in many cell types and is a fundamental component of mitochondria morphology, which is balanced between fusion and fission. Mitochondrial dysfunction is a major cause of neuronal death in degenerative disorders, such as Parkinson's disease, and can be triggered by an imbalance in the dynamic equilibrium between mitochondrial fission and fusion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MFN1