Mitochondrial ribosomal protein L11 Antibody - BSA Free

Western Blot: Mitochondrial ribosomal protein L11 Antibody [NBP3-06019] - Anti-MRPL11 rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary: Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:21 kDa Observed band size:21 kDa

Immunohistochemistry-Paraffin: Mitochondrial ribosomal protein L11 Antibody [NBP3-06019] - Immunochemical staining of human MRPL11 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: Mitochondrial ribosomal protein L11 Antibody [NBP3-06019] - Immunochemical staining of human MRPL11 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: Mitochondrial ribosomal protein L11 Antibody [NBP3-06019] - MRPL11 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-MRPL11 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody: Anti-MRPL11 rabbit polyclonal antibody, at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa Observed band size :21 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Mitochondrial ribosomal protein L11 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Mitochondrial ribosomal protein L11 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPL11
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Mitochondrial ribosomal protein L11 Antibody - BSA Free

• L11MT
• MGC111024
• mitochondrial ribosomal protein L11
• MRP-L11,39S ribosomal protein L11, mitochondrial

Background

MRPL11, also known as 39 S ribosomal protein L11, mitochondrial, consists of a 20.7 kDa, a 18.2 kDa, and a 19.5 kDa isoform and is utilized in mitochondrial ribosomes during protein synthesis. Diseases and disorders such as pneumonia, malaria, mycobacterium tuberculosis, and intrahepatic cholangiocarcinoma are being research with this protein. The protein interacts in the translational control pathway with ICT1, RPL26, MRPL2, RPL26L1, and MRPL3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPL11