MRPL12 Antibody (3B12-1A3) - Azide and BSA Free

Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody (M01), clone 3B12-1A3.Lane 1: MRPL12 transfected lysate(21.3 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of monoclonal antibody to MRPL12 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. Antibody concentration 3 ug/ml.

Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi ( Cat # H00006182-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody (M01), clone 3B12-1A3 (Cat # H00006182-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - MRPL12 monoclonal antibody (M01), clone 3B12-1A3 Analysis of MRPL12 expression in COLO 320 HSR.

ELISA: MRPL12 Antibody (3B12-1A3) [H00006182-M01] - Detection limit for recombinant GST tagged MRPL12 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, KD
• Clone: 3B12-1A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MRPL12 Antibody (3B12-1A3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: MRPL12 (AAH02344, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLPAAARPLWGPCLGLRAAAFRLARRQVPCVCAVRHMRSSGHQRCEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLVPMGGVMSGAVPAAAAQEAVEEDIPIAKERTHFTVRLTEAKPVDKVKLIKEIKNYIQGINLVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE
• Specificity: MRPL12 - mitochondrial ribosomal protein L12
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRPL12
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MRPL12 Antibody (3B12-1A3) - Azide and BSA Free

• 5c5-2
• FLJ60124
• L12mt
• MGC8610
• mitochondrial ribosomal protein L12
• MRP-L12
• MRP-L31/34
• MRPL7
• MRPL7/L12
• RPML1239S ribosomal protein L12, mitochondrial

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MRPL12 Antibody (H00006182-M01)(7)

Publications using H00006182-M01

• Xingzhao J, Lingju C, Dun S et al. MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells. iScience. 2023-04-19 [PMID: 37182101]
• KOnig T, Nolte H, Aaltonen MJ et al. MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control Nature cell biology 2021-12-01 [PMID: 34873283] (SR Microscopy)
• Mari A, Irina A, Tim K et al. Serine palmitoyltransferase assembles at ER-mitochondria contact sites. Life Sci Alliance. 2021-11-16 [PMID: 34785538]
• Richman TR, Davies SM, Shearwood AM et al. A bifunctional protein regulates mitochondrial protein synthesis. Nucleic Acids Res. 2014-01-31 [PMID: 24598254]
• Antonicka H, Sasarman F, Nishimura T et al. The Mitochondrial RNA-Binding Protein GRSF1 Localizes to RNA Granules and Is Required for Posttranscriptional Mitochondrial Gene Expression. Cell Metab 2013-03-05 [PMID: 23473033] (ICC/IF, Human)
• Chen Y, Cairns R, Papandreou I, et al. Oxygen consumption can regulate the growth of tumors, a new perspective on the warburg effect. PLoS One 4(9):e7033. 2009-09-15 [PMID: 19753307]
• Wang Z, Cotney J, Shadel GS. Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression. J Biol Chem;282(17):12610-8. 2007-04-27 [PMID: 17337445]

Bioinformatics

• Gene Symbol: MRPL12
• Entrez:
  • 6182(Human)
• OMIM:
  • 602375(Human)
• Uniprot:
  • AAH02344(Human)