MIST1 Antibody (6E8/A12/C11P1)

Immunohistochemistry: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478] - Mist1 expression can be observed in mouse gastric chief cells(red). image is courtesy of customer review.

Immunohistochemistry-Paraffin: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478] - Staining of mouse pancreas tissues. The antibody gives nuclear staining in acinar cells but not in duct or islet cells.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: 6E8/A12/C11P1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MIST1 Antibody (6E8/A12/C11P1) Summary

• Immunogen: Mouse Mist1, C-terminus residues 175-197.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BHLHA15
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:100 - 1:500
• Application Notes: The antibody gives nuclear staining in acinar cells but not in duct or islet cells. IHC reported in a verified customer review.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MIST1 Antibody (6E8/A12/C11P1)

• basic helix-loop-helix domain containing, class B, 8
• basic helix-loop-helix family, member a15
• bHLHa15
• BHLHB8
• class A basic helix-loop-helix protein 15
• Class B basic helix-loop-helix protein 8
• class II bHLH protein MIST1
• MIST1MIST-1
• Muscle, intestine and stomach expression 1

Background

MIST1 plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblastpopulations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. Mayalso negatively regulate bHLH-mediated t

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MIST1 Antibody (NBP2-22478)(4)

Publications using NBP2-22478

• Guan L, Viswanathan V, Jiang Y et Al. Tert-expressing cells contribute to salivary gland homeostasis and tissue regeneration after radiation therapy Genes Dev 2024-07-12 [PMID: 38997156]
• He J, Nascakova Z, Leary P et al. Inactivation of the tumor suppressor gene Apc synergizes with H. pylori to induce DNA damage in murine gastric stem and progenitor cells Science advances 2023-11-17 [PMID: 37967175] (ICC/IF, Mouse)
  
  Details:
  1:100 dilution
• Zhou H, Du Y, Wei X et al. DDX56 transcriptionally activates MIST1 to facilitate tumorigenesis of HCC through PTEN-AKT signaling Theranostics 2022-08-15 [PMID: 36168636] (Immunohistochemistry, Immunohistochemistry-Frozen)
• Sayols S, Klassek J, Werner C et al. Signalling codes for the maintenance and lineage commitment of embryonic gastric epithelial progenitors Development 2020-01-28 [PMID: 32878924] (Immunohistochemistry, Immunohistochemistry-Frozen)

Review for MIST1 Antibody (NBP2-22478) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Feng Li ICC Mouse 05/11/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: mouse stomach
• Species: Mouse
• Lot: RH223596

Bioinformatics

• Gene Symbol: BHLHA15
• Entrez:
  • 168620(Human)
• Uniprot:
  • Q7RTS1(Human)