TFEB Antibody (S1)

Images

 
Western Blot: TFEB Antibody (S1) [H00007942-M01] - TFEB monoclonal antibody (M01), clone S1. Analysis of TFEB expression in rat brain.
Immunocytochemistry/ Immunofluorescence: TFEB Antibody (S1) [H00007942-M01] - Analysis of monoclonal antibody to TFEB on HeLa cell. Antibody concentration 20 ug/ml.
Western Blot: TFEB Antibody (S1) [H00007942-M01] - TFEB monoclonal antibody (M01), clone 3E1-G6 Western Blot analysis of TFEB expression in Jurkat ( Cat # L017V1 ).
ELISA: TFEB Antibody (S1) [H00007942-M01] - TFEB Antibody (3E1-G6) [H00007942-M01] - Detection limit for recombinant GST tagged TFEB is approximately 0.03ng/ml as a capture antibody.
TFEB expression reduces PHF1 levels in the P301S model of tauopathy, and significantly attenuates the loss of neuronal and synaptic markers in the hippocampus. A) Lysates were prepared from the hippocampal regions of ...read more
G-TPP but not 17-AAG induces Parkin translocation(A, B) HeLa cells stably expressing EGFP-Parkin were treated with 12 different concentrations of G-TPP or 17-AAG in a dose-response format for 2 or 6 h before adding (A) ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, WB
Clone
S1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

TFEB Antibody (S1) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
TFEB (AAH32448, 1 a.a. ~ 476 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYINPEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAELAQQVVKQELPSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPGHGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEASKASSRRSSFSMEEGDVL
Specificity
TFEB - transcription factor EB
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TFEB
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunoblotting
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA. Use in IHC-P reported in scientific literature (PMID:35897713).
Reviewed Applications
Read 1 Review rated 3
using
H00007942-M01 in the following applications:

Publications
Read Publications using
H00007942-M01 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TFEB Antibody (S1)

  • ALPHATFEB
  • BHLHE35
  • Class E basic helix-loop-helix protein 35
  • T-cell transcription factor EB
  • TCFEB
  • TCFEBbHLHe35
  • TFEB
  • transcription factor EB

Background

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TFEB Antibody (H00007942-M01)(6)

Review for TFEB Antibody (H00007942-M01) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using H00007942-M01:
Filter by Applications
Simple Western
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
Simple Western TFEB H00007942-M01
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3
reviewed by:
Verified Customer
Simple Western Rat 08/18/2017
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Summary

ApplicationSimple Western
Sample Testedcell line, whole cell lysate
SpeciesRat
LotG5031-S1

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TFEB Antibody (H00007942-M01) (0)

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Secondary Antibodies

 

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By Christina Towers, PhD. In the last 20 years, epigenetic regulation has become front and center for almost all fields of biology and its role in diseases like cancer and neurodegeneration are being heavily studi...  Read full blog post.

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Verified Customer
08/18/2017
Application: Simple Western
Species: Rat

Bioinformatics

Gene Symbol TFEB
Entrez
OMIM
Uniprot