MiRP2 Antibody

Immunohistochemistry-Paraffin: MiRP2 Antibody [NBP1-49885] - 3.75ug/ml staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MiRP2 Antibody Summary

• Immunogen: Peptide with sequence C-PDNQTEERRASLP corresponding to internal region according to NP_005463.1.
• Epitope: PDNQTEERRASLP - Internal region
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: KCNE3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:1000
  • Western Blot detection limit 1:1000
• Application Notes: Western blot: Preliminary experiments in Human, Mouse and Rat Skeletal Muscle lysates gave no specific signal but low background
  IHC: Paraffin embedded Human Kidney.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine, Bovine, Porcine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MiRP2 Antibody

• cardiac voltage-gated potassium channel accessory subunit
• DKFZp781H21101
• HOKPPMGC102685
• HYPP
• MGC129924
• Minimum potassium ion channel-related peptide 2
• MinK-related peptide 2
• MiRP2
• Potassium channel subunit beta MiRP2
• potassium voltage-gated channel subfamily E member 3
• potassium voltage-gated channel, Isk-related family, member 3
• voltage-gated K+ channel subunit MIRP2

Background

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, isk-related subfamily. This member is a type I membrane protein, and a beta subunit that assembles with a potassium channel alpha-subunit to modulate the gating kinetics and enhance stability of the multimeric complex. This gene is prominently expressed in the kidney. A missense mutation in this gene is associated with hypokalemic periodic paralysis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MiRP2 Antibody (NBP1-49885)(1)

Publication using NBP1-49885

• Ohno S, Toyoda F, Zankov DP et al. Novel KCNE3 mutation reduces repolarizing potassium current and associated with long QT syndrome. Hum Mutat 2009-04-01 [PMID: 19306396]

Bioinformatics

• Gene Symbol: KCNE3
• Entrez:
  • 10008(Human)
  • 57442(Mouse)
  • 63883(Rat)
• Uniprot:
  • NP_005463.1(Human)