Potassium Channel Kv3.1 [p Ser503] Antibody - Azide Free

Immunohistochemistry: Kv3.1 [p Ser503] Antibody [NB300-279] - IHC staining of medial nucleus of the trapezoid body (MNTB) cells with the phospho-Ser503 Kv3.1 subunit antibody. The left panel shows control cells. The right panel shows cells that have been exposed to the protein kinase C activator PMA.

• Reactivity: Mu, Rt, Ha
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Potassium Channel Kv3.1 [p Ser503] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit Potassium Channel Kv3.1 [p Ser503] Antibody - Azide Free (NB300-279) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Potassium Channel Kv3.1 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser503 conjugated to KLH. Accession # P25122
• Modification: p Ser503
• Specificity: Specific for endogenous levels of the ~100 kDa Kv3.1 voltage-gated potassium channel protein phosphorylated at Ser503.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNC1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:400
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:1000
  • Western Blot 1:1000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Tested in Rat and Mouse.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Potassium Channel Kv3.1 [p Ser503] Antibody - Azide Free

• FLJ41162
• FLJ42249
• FLJ43491
• KCNC1
• KV3.1
• KV4
• MGC129855
• NGK2
• Potassium Channel Kv3.1
• potassium voltage-gated channel subfamily C member 1
• potassium voltage-gated channel, Shaw-related subfamily, member 1
• voltage-gated potassium channel protein KV3.1
• Voltage-gated potassium channel subunit Kv3.1
• Voltage-gated potassium channel subunit Kv4

Background

Voltage-gated K+ channels are important determinants of neuronal membrane excitability. Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types (Maletic-Savatic et al., 1995; Pongs, 1999; Blaine and Ribera, 1998; Burger and Ribera, 1996). The Kv3.1 potassium channel is expressed at high levels in neurons that characteristically fire rapid trains of action potentials (Gan et al., 1999). Particularly high levels of this channel are found in neurons of the auditory brainstem. These neurons appear to participate in neural circuits that determine the intensity and timing of auditory stimuli and use this information to determine the location of sounds in space (von Hehn et al., 2004).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Potassium Channel Kv3.1 Antibody (NB300-279)(6)

Publications using NB300-279

• von Hehn CA, Bhattacharjee A, Kaczmarek LK. Loss of Kv3.1 tonotopicity and alterations in cAMP response element-binding protein signaling in central auditory neurons of hearing impaired mice. J Neurosci;24:1936-1940. 2004-01-01 [PMID: 14985434]
• Gan L, Hahn SJ, Kaczmarek LK. Cell type-specific expression of the Kv3.1 gene is mediated by a negative element in the 5' untranslated region of the Kv3.1 promoter. J Neurochem 73:1350-1362. 1999-01-01 [PMID: 10501178]
• Pongs O. Voltage-gated potassium channels: from hyperexcitability to excitement. FEBS Lett 452:31-35. 1999-01-01 [PMID: 10376673]
• Blaine JT, Ribera AB. Heteromultimeric potassium channels formed by members of the Kv2 subfamily. J. Neurosci 18:9585-9593. 1998-01-01 [PMID: 9822719]
• Burger C, Ribera AB. Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development. J Neurosci 16:1412-1421. 1996-01-01 [PMID: 8778292]
• Maletic-Savatic M, Lenn NJ, Trimmer JS. Differential spatiotemporal expression of K+ channel polypeptides in rat hippocampal neurons developing in situ in vitro. J Neurosci 15:3840-3851. 1995-01-01 [PMID: 7751950]

Bioinformatics

• Gene Symbol: KCNC1
• Entrez:
  • 16502(Mouse)
  • 25327(Rat)
• Uniprot:
  • P25122(Rat)