Mindin/Spondin-2 Antibody

Western Blot: Mindin/Spondin-2 Antibody [NBP1-52031] - NBP1-52031 (1ug/ml) staining of Human Prostate lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Mindin/Spondin-2 Antibody Summary

• Immunogen: Peptide with sequence C-RKNQYVSNGLRDFA corresponding to internal region according to NP_036577.1.
• Epitope: C-RKNQYVSNGLRDFA
• Specificity: Reported variants NP_036577.1 and NP_001121797.1 represent identical protein.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SPON2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in human prostate lysates (calculated MW of 35.8 kDa band according to NP_036577.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Mindin/Spondin-2 Antibody

• Differentially expressed in cancerous and non-cancerous lung cells 1
• DIL1
• DIL-1
• DIL1FLJ34460
• DKFZp686G21139
• FLJ16313
• FLJ22401
• Mindin
• M-SPONDIN
• RG-1
• SPON2
• Spondin 2
• spondin 2, extracellular matrix protein
• spondin-2

Background

Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens . Binds bacterial lipopolysaccharide (LPS)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Mindin/Spondin-2 Antibody (NBP1-52031)(1)

Publication using NBP1-52031

• Manda R, Kohno T, Matsuno Y et al. Identification of genes (SPON2 and C20orf2) differentially expressed between cancerous and noncancerous lung cells by mRNA differential display. Genomics 1999-10-01 [PMID: 10512675]

Bioinformatics

• Gene Symbol: SPON2
• Entrez:
  • 10417(Human)
• Uniprot:
  • NP_036577.1(Human)