MINA Antibody (753002) [Alexa Fluor® 488]

• Applications: Flow
• Clone: 753002
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

MINA Antibody (753002) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human MINA
  Met1-Gly192
  Accession # Q8IUF8
• Specificity: Detects human MINA in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse MINA is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MINA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

MINA (Myc-induced Nuclear Antigen), also known as Mina53, is a 52-54 kDa member of both the MINA53/NO66 and Jumonji C family of proteins. It expression is associated with proliferating cells, and it has been found in cytoplasm, nucleus and particularly nucleoli. MINA appears to be induced by c-myc, and synthesized by spermatogonia, select epithelium, naïve T cells and select cancer cells. When expressed, MINA is reported to regulate expression of genes such as HGF, EGF-R, IL-4, and Relm beta , the latter through the influence of TGF-beta . It may exert its regulatory activity through an intrinsic histone demethylase function. Human MINA is 465 amino acids (aa) in length. It possesses one cupin (or enzyme‑associated) region (aa 52-364) that contains a JmjC domain (aa 139-271). There are three potential isoform variants that show either a seven aa substitution for aa 255‑261, an 18 aa substitution for aa 263-465, or a deletion of Glu297. Over aa 2-192, human MINA shares 82% aa sequence identity with mouse MINA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MINA