MIF4GD Antibody (OTI4C11)

Western Blot: MIF4GD Antibody (4C11) [NBP2-01876] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIF4GD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIF4GD.

Immunocytochemistry/Immunofluorescence: MIF4GD Antibody (4C11) [NBP2-01876] Staining of COS7 cells transiently transfected by pCMV6-ENTRY MIF4GD.

Flow Cytometry: MIF4GD Antibody (4C11) [NBP2-01876] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MIF4GD antibody, and then analyzed by flow cytometry.

Immunocytochemistry/Immunofluorescence: MIF4GD Antibody (4C11) [NBP2-01876] - Immunofluorescent staining of HeLa cells using anti-MIF4GD mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF
• Clone: OTI4C11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.78 mg/ml

MIF4GD Antibody (OTI4C11) Summary

• Description: Novus Biologicals Mouse MIF4GD Antibody (OTI4C11) (NBP2-01876) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human MIF4GD(NP_065730) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MIF4GD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 29.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.78 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MIF4GD Antibody (OTI4C11)

• AD023
• hSLIP1
• MGC45027
• MIF4G domain containing
• MIFD
• SLBP (stem loop binding protein)-interacting protein 1
• SLBP-interacting protein 1
• SLIP1MIF4G domain-containing protein

Background

This gene encodes a protein which interacts with the N-terminus of the stem-loop binding protein (SLBP) and the 3' end of histone mRNA. This interaction facilitates the activation of histone mRNA translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MIF4GD
• Entrez:
  • 57409(Human)