Western Blot: EPLIN Antibody [NB100-2305] - Detection of Human EPLIN by Western Blot. Samples: A) Whole cell lysate (50 mcg) from DU145 and SKBR3 cells. B) EPLIN that was immunoprecipitated from 500 mcg of whole cell ...read more
Hu, Bovine, Equine, Primate, Mouse, Rabbit, Rat, Chinese Hamster, Rhesus MacaqueSpecies Glossary
The immunogen recognized by this antibody maps to a region between residues 700 and the C-terminus (residue 759) of human epithelial protein lost in neoplasm beta using the numbering given in entry NP_057441.1 (GeneID 51474)
Mouse (100%), Rat (100%), Rhesus Macaque (100%), Chinese Hamster (100%), Rabbit (100%), Primate (100%), Bovine (100%), Equine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
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Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque, African elephant, Naked mole rat and Northern white-cheeked gibbon.
Alternate Names for EPLIN Antibody
Epithelial protein lost in neoplasm
EPLINepithelial protein lost in neoplasm beta
LIM domain and actin binding 1
SREBP3LIM domain and actin-binding protein 1
sterol regulatory element binding protein 3
EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles (Maul et al., 2003).[supplied by OMIM]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for EPLIN Antibody (NB100-2305). (Showing 1 - 1 of 1 FAQs).
What's the epitope and where does it bind on EPLIN? Does NB100-2305 recognize only the alpha and the beta forms, and not other isoforms(there are 4 in total)? I get multiple bands (more than 2) and just want to make sure which band corresponds to which isoforms.
Our EPLIN antibody with catalogue number NB100-2305 was raised against an immunogen derived from amino acids 700-759 of human EPLIN (NP_057441.1). Unfortunately we do not epitope map our antibodies and so I am unable to provide you with any further information regarding the specific binding of NB100-2305 to its target. Our Western blot data, as you rightly point out, shows the antibody to bind to EPLIN alpha and EPLIN beta in lysates from DU145 and SKBR3 cells. We have not yet tested its binding to the other two EPLIN isoforms. According to the Uniprot listing, the theoretical molecular weight of isoform beta is 85kDa. isoform alpha is 67kDa, isoform 3 is 51kDa and isoform 4 is 85kDa. The EPLIN protein has multiple phosphorylation sites, so the phosphorylation status may also have an impact on the size at which your bands are running.