Western Blot: EPLIN Antibody [NB100-2305] - Whole cell lysate from MCF7 (5, 15 and 50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPLIN antibody used for WB at 0.4 ug/ml. ...read more
Western Blot: EPLIN Antibody [NB100-2305] - Detection of Human EPLIN by Western Blot. Samples: A) Whole cell lysate (50 mcg) from DU145 and SKBR3 cells. B) EPLIN that was immunoprecipitated from 500 mcg of whole cell ...read more
Independent Antibodies: Western Blot: EPLIN Antibody [NB100-2305] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF7 cells prepared using NETN lysis buffer. Antibodies: ...read more
The immunogen recognized by this antibody maps to a region between residues 700 and the C-terminus (residue 759) of human epithelial protein lost in neoplasm beta using the numbering given in entry NP_057441.1 (GeneID 51474)
Immunogen affinity purified
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FAQs for EPLIN Antibody (NB100-2305). (Showing 1 - 1 of 1 FAQs).
What's the epitope and where does it bind on EPLIN? Does NB100-2305 recognize only the alpha and the beta forms, and not other isoforms(there are 4 in total)? I get multiple bands (more than 2) and just want to make sure which band corresponds to which isoforms.
Our EPLIN antibody with catalogue number NB100-2305 was raised against an immunogen derived from amino acids 700-759 of human EPLIN (NP_057441.1). Unfortunately we do not epitope map our antibodies and so I am unable to provide you with any further information regarding the specific binding of NB100-2305 to its target. Our Western blot data, as you rightly point out, shows the antibody to bind to EPLIN alpha and EPLIN beta in lysates from DU145 and SKBR3 cells. We have not yet tested its binding to the other two EPLIN isoforms. According to the Uniprot listing, the theoretical molecular weight of isoform beta is 85kDa. isoform alpha is 67kDa, isoform 3 is 51kDa and isoform 4 is 85kDa. The EPLIN protein has multiple phosphorylation sites, so the phosphorylation status may also have an impact on the size at which your bands are running.
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