EPLIN Antibody

Images

 
Western Blot: EPLIN Antibody [NB100-2305] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-EPLIN ...read more
Western Blot: EPLIN Antibody [NB100-2305] - Detection of Human EPLIN by Western Blot. Samples: A) Whole cell lysate (50 mcg) from DU145 and SKBR3 cells. B) EPLIN that was immunoprecipitated from 500 mcg of whole cell ...read more
Western Blot: EPLIN Antibody [NB100-2305] - Samples: Whole cell lysate (50 ug) from MCF7, HeLa, and 293T cells prepared using NETN lysis buffer. Antibody : Affinity purified rabbit anti-EPLIN antibody used for WB at 0.4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

EPLIN Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 700 and the C-terminus (residue 759) of human epithelial protein lost in neoplasm beta using the numbering given in entry NP_057441.1 (GeneID 51474)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIMA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 2-10 ug/mg lysate
Positive Control
EPLIN Lysate (NBL1-12533)
Publications
Read Publications using
NB100-2305 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EPLIN Antibody

  • 1110021C24Rik
  • Epithelial protein lost in neoplasm
  • EPLINepithelial protein lost in neoplasm beta
  • FLJ38853
  • LIM domain and actin binding 1
  • MGC131726
  • SREBP3LIM domain and actin-binding protein 1
  • sterol regulatory element binding protein 3

Background

EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles (Maul et al., 2003).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-31357
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP1-90363
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
H00009275-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP2-30627
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF497
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
H00001164-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NB600-1293
Species: Hu, Mu, Rt, Am, Bv, Ca, Ch, Tr
Applications: WB, ICC/IF, IHC, IHC-Fr
H00010097-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-89726
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-49118
Species: Hu
Applications: IHC, IHC-P
NB200-109
Species: Hu, Mu
Applications: WB, ChIP, Flow, GS, ICC/IF, IP, CyTOF-ready
AF5458
Species: Hu
Applications: WB, ICC
NBP1-83976
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-17058
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for EPLIN Antibody (NB100-2305)(4)

Reviews for EPLIN Antibody (NB100-2305) (0)

There are no reviews for EPLIN Antibody (NB100-2305). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EPLIN Antibody (NB100-2305). (Showing 1 - 1 of 1 FAQs).

  1. What's the epitope and where does it bind on EPLIN? Does NB100-2305 recognize only the alpha and the beta forms, and not other isoforms(there are 4 in total)? I get multiple bands (more than 2) and just want to make sure which band corresponds to which isoforms.
    • Our EPLIN antibody with catalogue number NB100-2305 was raised against an immunogen derived from amino acids 700-759 of human EPLIN (NP_057441.1). Unfortunately we do not epitope map our antibodies and so I am unable to provide you with any further information regarding the specific binding of NB100-2305 to its target. Our Western blot data, as you rightly point out, shows the antibody to bind to EPLIN alpha and EPLIN beta in lysates from DU145 and SKBR3 cells. We have not yet tested its binding to the other two EPLIN isoforms. According to the Uniprot listing, the theoretical molecular weight of isoform beta is 85kDa. isoform alpha is 67kDa, isoform 3 is 51kDa and isoform 4 is 85kDa. The EPLIN protein has multiple phosphorylation sites, so the phosphorylation status may also have an impact on the size at which your bands are running.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional EPLIN Products

Bioinformatics Tool for EPLIN Antibody (NB100-2305)

Discover related pathways, diseases and genes to EPLIN Antibody (NB100-2305). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EPLIN Antibody (NB100-2305)

Discover more about diseases related to EPLIN Antibody (NB100-2305).
 

Pathways for EPLIN Antibody (NB100-2305)

View related products by pathway.

PTMs for EPLIN Antibody (NB100-2305)

Learn more about PTMs related to EPLIN Antibody (NB100-2305).

Blogs on EPLIN

There are no specific blogs for EPLIN, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our EPLIN Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol LIMA1
Entrez
Uniprot