EPLIN Antibody

Images

 
Western Blot: EPLIN Antibody [NB100-2305] - Whole cell lysate from MCF7 (5, 15 and 50 ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-EPLIN antibody used for WB at 0.4 ug/ml. ...read more
Independent Antibodies: Immunoprecipitation: EPLIN Antibody [NB100-2305] - Detection of human EPLIN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

Order Details

EPLIN Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 700 and the C-terminus (residue 759) of human epithelial protein lost in neoplasm beta using the numbering given in entry NP_057441.1 (GeneID 51474)
Predicted Species
Mouse (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LIMA1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • Immunohistochemistry 1:10-1:500
  • Immunoprecipitation 2-10 ug/mg lysate
Application Notes
IHC reactivity reported in (PMID: 21625216).
Control
EPLIN Overexpression Lysate
Publications
Read Publications using
NB100-2305 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for EPLIN Antibody

  • 1110021C24Rik
  • Epithelial protein lost in neoplasm
  • EPLINepithelial protein lost in neoplasm beta
  • FLJ38853
  • LIM domain and actin binding 1
  • MGC131726
  • SREBP3LIM domain and actin-binding protein 1
  • sterol regulatory element binding protein 3

Background

EPLIN is a cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles (Maul et al., 2003).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EPLIN Antibody (NB100-2305)(4)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EPLIN Antibody (NB100-2305). (Showing 1 - 1 of 1 FAQs).

  1. What's the epitope and where does it bind on EPLIN? Does NB100-2305 recognize only the alpha and the beta forms, and not other isoforms(there are 4 in total)? I get multiple bands (more than 2) and just want to make sure which band corresponds to which isoforms.
    • Our EPLIN antibody with catalogue number NB100-2305 was raised against an immunogen derived from amino acids 700-759 of human EPLIN (NP_057441.1). Unfortunately we do not epitope map our antibodies and so I am unable to provide you with any further information regarding the specific binding of NB100-2305 to its target. Our Western blot data, as you rightly point out, shows the antibody to bind to EPLIN alpha and EPLIN beta in lysates from DU145 and SKBR3 cells. We have not yet tested its binding to the other two EPLIN isoforms. According to the Uniprot listing, the theoretical molecular weight of isoform beta is 85kDa. isoform alpha is 67kDa, isoform 3 is 51kDa and isoform 4 is 85kDa. The EPLIN protein has multiple phosphorylation sites, so the phosphorylation status may also have an impact on the size at which your bands are running.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Contact Information

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Bioinformatics

Gene Symbol LIMA1
Entrez
Uniprot