MICL/CLEC12A Antibody (812303) [Unconjugated]

Mouse splenocytes were stained with either (A) Rat Anti-Mouse MICL/CLEC12A Monoclonal Antibody (Catalog # MAB2950) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Goat Anti-Rat PE-conjugated secondary antibody (Catalog # F0105B) and Rat Anti-Mouse CD11c APC-conjugated Monoclonal Antibody (Catalog # FAB69501A). Cells were first gated on Rat Anti-Mouse CD8 alpha Alexa Fluor® 488-conjugated Monoclonal Antibody-positive cells (Catalog # FAB116G, not shown) . View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 812303
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

MICL/CLEC12A Antibody (812303) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MICL/CLEC12A with an N-terminal 9-His tag
  Thr67-Arg267
  Accession # Q504P2
• Specificity: Detects mouse MICL/CLEC12A in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICL/CLEC12A Antibody (812303) [Unconjugated]

• CD371
• CLEC12A
• CLL1
• CLL-1
• C-type lectin domain family 12, member A
• DCAL-2
• MICL

Background

MICL, also known as killer cell lectin-like receptor 1 (KLR1), is an inhibitory C-type lectin-like receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). It is a75 kDa type II membrane protein that is expressed on neutrophils, NK cells, monocytes and dendritic cells. The amino acid sequence of mouse MICL extracellular domain is 53%, 52% and 58% identical to that of human, bovine and canine MICL, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q504P2()