MICALL1 Antibody

Images

 
Western Blot: MICALL1 Antibody [H00085377-B01P] - Analysis of MICALL1 expression in transfected 293T cell line by MICALL1 polyclonal antibody. Lane 1: MICAL-L1 transfected lysate(94.93 KDa). Lane 2: Non-transfected ...read more
Immunocytochemistry/ Immunofluorescence: MICALL1 Antibody [H00085377-B01P] - GRAF/WDR44 label a subset of tubular endosomes. Insets show magnifications of the boxed areas. Scale bars: 10 um; scale bars of insets: 2 um. ...read more
Immunohistochemistry-Paraffin: MICALL1 Antibody [H00085377-B01P] - IHC staining of MICALL1 in human prostate cancer using DAB with hematoxylin counterstain.
Western Blot: MICALL1 Antibody [H00085377-B01P] - Analysis of MICALL1 expression in HepG2.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MICALL1 Antibody Summary

Immunogen
MICAL-L1 (AAI48805.1, 1 a.a. - 863 a.a.) full-length human protein. MAGPRGALLAWCRRQCEGYRGVEIRDLSSSFRDGLAFCAILHRHRPDLLDFDSLSKDNVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYNHFCSPGQAGVSPPRKGLAPCSPPSVAPTPVEPEDVAQGEELSSGSLSEQGTGQTPSSTCAACQQHVHLVQRYLADGRLYHRHCFRCRRCSSTLLPGAYENGPEEGTFVCAEHCARLGPGTRSGTRPGPFSQPKQQHQQQLAEDAKDVPGGGPSSSAPAGAEADGPKASPEARPQIPTKPRVPGKLQELASPPAGRPTPAPRKASESTTPAPPTPRPRSSLQQENLVEQAGSSSLVNGRLHELPVPKPRGTPKPSEGTPAPRKDPPWITLVQAEPKKKPAPLPPSSSPGPPSQDSRQVENGGTEEVAQPSPTASLESKPYNPFEEEEEDKEEEAPAAPSLATSPALGHPESTPKSLHPWYGITPTSSPKTKKRPAPRAPSASPLALHASRLSHSEPPSATPSPALSVESLSSESASQTAGAELLEPPAVPKSSSEPAVHAPGTPGNPVSLSTNSSLASSGELVEPRVEQMPQASPGLAPRTRGSSGPQPAKPCSGATPTPLLLVGDRSPVPSPGSSSPQLQVKSSCKENPFNRKPSPAASPATKKATKGSKPVRPPAPGHGFPLIKRKVQADQYIPEEDIHGEMDTIERRLDALEHRGVLLEEKLRGGLNEGREDDMLVDWFKLIHEKHLLVRRESELIYVFKQQNLEQRQADVEYELRCLLNKPEKDWTEEDRAREKVLMQELVTLIEQRNAIINCLDEDRQREEEEDKMLEAMIKKKEFQREAEPEGKKKGKFKTMKMLKLLGNKRDAKSKSPRDKS
Specificity
MICAL-L1 - MICAL-like 1,
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
MICALL1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. Antibody is also useful for Immunohistochemistry on paraffin-embedded sections. Immunocytochemistry/Immunofluorescence, Immunohistochemistry and Immunoprecipitation were reported in scientific literature, but Immunocytochemistry/Immunofluorescence has been experiencing lot-to-lot variations. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID: 24019528).
Publications
Read Publications using
H00085377-B01P in the following applications:

  • 7 publications
  • IHC
    1 publication
  • IP
    1 publication
  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MICALL1 Antibody

  • DKFZp686M2226
  • KIAA1668
  • MICAL-L1
  • MICAL-like 1
  • MICAL-like protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MICALL1 Antibody (H00085377-B01P)(19)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.


Filter By Application
ICC/IF
(7)
IHC
(1)
IP
(1)
WB
(6)
All Applications
Filter By Species
Human
(14)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using H00085377-B01P Applications Species
Lucken-Ardjomande HAsler S, Vallis Y, Pasche M, McMahon HT GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR delta, F508 J. Cell Biol. May 4 2020 [PMID: 32344433] (WB, Human) WB Human
Siddiqa A, Massimi P, Pim D, Banks L Diverse Papillomavirus Types Induce Endosomal Tubulation Front Cell Infect Microbiol May 28 2019 [PMID: 31192164] (ICC/IF, Human) ICC/IF Human
Fukuda M Rab10 regulates tubular endosome formation through KIF13A/B motors. J Cell Sci. 2019 Feb 19 [PMID: 30700496]
Xie S, Reinecke JB, Farmer T et al. Vesicular trafficking plays a role in centriole disengagement and duplication Mol. Biol. Cell Nov 1 2018 [PMID: 30188792] (WB, Human) WB Human
Finicle BT, Ramirez MU, Liu G et al. Sphingolipids inhibit endosomal recycling of nutrient transporters by inactivating ARF6 J. Cell. Sci. Jun 25 2018 [PMID: 29848659] (Human) Human
Mukadam AS, Breusegem SY, Seaman MNJ. Analysis of novel endosome-to-Golgi retrieval genes reveals a role for PLD3 in regulating endosomal protein sorting and amyloid precursor protein processing Cell. Mol. Life Sci. 2018 Jan 24 [PMID: 29368044] (WB, Human) WB Human
Siddiqa A, Massimi P, Pim D et al. Human Papillomavirus 16 infection induces VAP-dependent endosomal tubulation J. Virol. 2018 Jan 10 [PMID: 29321327] (Human) Human
Vidal-Quadras M, Holst MR, Francis MK et al. Endocytic turnover of Rab8 controls cell polarization. J. Cell. Sci. Mar 15 2017 [PMID: 28137756] (WB, Human) WB Human
Bahl K, Xie S, Spagnol G et al. EHD3 is Required for Tubular Recycling Endosome Stabilization and an Asparagine-Glutamic Acid Residue Pair within its EH Domain Dictates its Selective Binding to NPF Peptides. J. Biol. Chem. May 4 2016 [PMID: 27189942] (Human) Human
Reinecke Jb, Katafiasz D, Naslavsky N, Caplan S. Novel Functions for the Endocytic Regulatory Proteins MICAL-L1 AnD EHD1 in Mitosis. Traffic. 2014 Oct 07 [PMID: 25287187] (WB, ICC/IF, Human) WB, ICC/IF Human
Show All 19 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MICALL1
Entrez
Uniprot