MICA/B Antibody (6D4)

Product Discontinued

MICA/B Antibody (6D4) Summary

• Immunogen: The immunogen was the full length version of the protein: MICA/MICB (6D4)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MICA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry < = 0.5 ug/10^6 cells in 100 ul
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Additional reported applications include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, and blocking of MIC mediated cytotoxicity. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 25510288)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for MICA/B Antibody (6D4)

• MHC class I chain-related protein A
• MHC class I chain-related protein B
• MIC
• MICA
• MICA/B
• MICB
• PERB11
• PERB11.1
• PERB11.2

Background

6D4 antibody reacts with a common epitope of the human nonclassical MHC class I chain-related protein A (MICA) and B (MICB), also known as PERB11.1 and PERB11.2. The MIC gene is located in MHC class I region. MICA/B are 65-75 kD stress-inducible glycoproteins with highly polymorphic. They are MHC class I-like transmembrane molecules that do not associate beta2-microglobulin and do not present peptides. MICA and MICB share 85% identify, and are mainly expressed on Intestinal epithelial cells, epithelial tumor cells, endothelial cells, fibroblasts, and IFN- alpha-stimulated dendritic cells. MIC molecules bind NKG2D, an activating receptor, and induce activation of NK cells, and subset of CD8+ alpha/ beta T cells and gamma/ delta T cells, as well as suppression of T cell proliferation. MICA/B recognition is involved in the regulation of tumor surveillance, viral infection and autoimmune diseases. The 6D4 antibody is able to block MIC mediated cytotoxicity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICA/B Antibody (NB100-78034)(2)

Publications using NB100-78034

• Guo M, Wu T, Wan L. Cytotoxic activity of allogeneic natural killer cells on U251 glioma cells in vitro. Mol Med Rep. 2016-05-06 [PMID: 27175912]
  
  Details:
  This citation used the PE version of this antibody.
• Cho H, Chung JY, Kim S et al. MICA/B and ULBP1 NKG2D ligands are independent predictors of good prognosis in cervical cancer. BMC Cancer 2014-12-15 [PMID: 25510288] (IHC-P, Human)

Bioinformatics

• Gene Symbol: MICA