MICA Antibody [Unconjugated]

MICA was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human MICA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1300) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MICA Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human MICA
  Ala23-Gln308
  Accession # NP_001170990
• Specificity: Detects human MICA in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MICA
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICA Antibody [Unconjugated]

• FLJ60820
• MGC111087
• MICA
• PERB11.1

Background

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are found within the Major Histocompatibility Complex on human chromosome 6. The MICA locus is highly polymorphic with more than 50 recognized human alleles. MICA is absent from most cells but is frequently expressed in epithelial tumors and can be induced by bacterial and viral infections. MICA is a ligand for human NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8⁺  alpha beta  T cells. Recognition of MICA by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

1. Groh, V. et al. (2001) Nature Immunol. 2:255.
2. Stephens, H. (2001) Trends Immunol. 22:378.
3. Bauer, S. et al. (1999) Science 285:727.
4. Groh, V. et al. (2002) Nature 419:734.
5. Steinle, A. et al. (2001) Immunogenetics 53:279.
6. Pende, D. et al. (2002) Cancer Res. 62:6178.
7. NKG2D and its Ligands (2002) http://www.RnDSystems.com

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICA Antibody (AF1300)(10)

Publications using AF1300

• MC Rittmann, S Hussung, LM Braun, RFU Klar, EA Biesel, S Fichtner-F, R Fritsch, UA Wittel, DA Ruess Plasma biomarkers for prediction of early tumor recurrence after resection of pancreatic ductal adenocarcinoma Scientific Reports, 2021-04-05;11(1):7499. 2021-04-05 [PMID: 33820913] (ICC, Human)
• DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging JCI Insight, 2019-06-11;5(0):. 2019-06-11 [PMID: 31184599] (IHC, Human)
• Boerman GH, van Ostaijen-ten Dam MM, Kraal KC et al. Role of NKG2D, DNAM-1 and natural cytotoxicity receptors in cytotoxicity toward rhabdomyosarcoma cell lines mediated by resting and IL-15-activated human natural killer cells. Cancer Immunol Immunother 5/1/2015 [PMID: 25854581]
• Herrmann , Julia, Berberich , Hannah, Hartmann , Jessica, Beyer , Steffen, Davies , Karen, Koch , Joachim Homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligands. J Biol Chem, 2013-11-25;289(2):765-77. 2013-11-25 [PMID: 24275655] (ELISA Development, Moth - Trichoplusia ni (Cabbage Looper))
• Annabelle Chauveau, Pierre Tonnerre, Angélique Pabois, Pierre-Jean Gavlovsky, Mathais Chatelais, Stéphanie Coupel et al. Endothelial Cell Activation and Proliferation Modulate NKG2D Activity by Regulating MICA Expression and Shedding Journal of Innate Immunity 1/1/2014 [PMID: 23860405]
• Li L, Chen A, Chaudhuri A, Kambham N, Sigdel T, Chen R, Sarwal MM Compartmental localization and clinical relevance of MICA antibodies after renal transplantation. Transplantation, 2010-02-15;89(3):312-9. 2010-02-15 [PMID: 20145522] (IHC-P, Human)
• Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells. Int. J. Cancer, 2009-04-01;124(7):1594-604. 2009-04-01 [PMID: 19089914] (Western Blot, Human)
• Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways. Mol. Immunol., 2008-07-26;45(15):3917-25. 2008-07-26 [PMID: 18657862] (IHC-P, Human)
• Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socie G, Toubert A An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation. J. Immunol., 2008-08-01;181(3):2227-37. 2008-08-01 [PMID: 18641363] (IHC-Fr, Human)
• Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2. Cancer Res., 2007-08-01;67(15):7458-66. 2007-08-01 [PMID: 17671216] (Western Blot, Human)

Review for MICA Antibody (AF1300) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 01/05/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23772752
• Species: Human

Bioinformatics

• Gene Symbol: MICA
• Uniprot:
  • NP_001170990()