MICA Antibody (159233) [Unconjugated]

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 159233
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MICA Antibody (159233) [Unconjugated] Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with human MICA
  Accession # Q29983
• Specificity: Detects human MICA in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human MICB is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MICA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human MICA Antibody (Catalog # MAB13002)
  ELISA Detection: Human MICA Biotinylated Antibody (Catalog # BAF1300)
  Standard: Recombinant Human MICA Fc Chimera (Catalog # 1300-MA)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MICA Antibody (159233) [Unconjugated]

• FLJ60820
• MGC111087
• MICA
• PERB11.1

Background

MHC class I Chain-related gene A (MICA) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MICA Antibody (MAB13002)(2)

Publications using MAB13002

• Carmen Campos-Silva, Yaiza Cáceres-Martell, Estela Sánchez-Herrero, Amaia Sandúa, Alexandra Beneitez-Martínez, Álvaro González et al. A simple immunoassay for extracellular vesicle liquid biopsy in microliters of non-processed plasma Journal of Nanobiotechnology 12/1/2022 [PMID: 35135541]
• Guerra S, Gonzalez JM, Climent N, Reyburn H, Lopez-Fernandez LA, Najera JL, Gomez CE, Garcia F, Gatell JM, Gallart T, Esteban M Selective induction of host genes by MVA-B, a candidate vaccine against HIV/AIDS. J. Virol., 2010-06-09;84(16):8141-52. 2010-06-09 [PMID: 20534857] (ELISA Development, ICC, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: MICA
• Uniprot:
  • Q29983()