Recombinant Human MIA Protein Summary
| Description |
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-131 of Human MIA Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGPMPKLADR KLCADQECSH PISMAVALQD YMAPDCRFLT IHRGQVVYVF SKLKGRGRLF WGGSVQGDYY GDLAARLGYF PSSIVREDQT LKPGKVDVKT DKWDFYCQ |
| Source |
E. coli |
| Protein/Peptide Type |
Recombinant Protein |
| Gene |
MIA |
| Purity |
>90%, by SDS-PAGE |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays. |
| Theoretical MW |
14.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
| Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer |
20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA |
| Preservative |
No Preservative |
| Concentration |
0.25 mg/ml |
| Purity |
>90%, by SDS-PAGE |
Alternate Names for Recombinant Human MIA Protein
Background
Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, includinggliomas
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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