MIA Antibody (294203) [Unconjugated]

MIA was detected in immersion fixed paraffin-embedded sections of human pancreas array using Mouse Anti-Human MIA Monoclonal Antibody (Catalog # MAB20501) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 294203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

MIA Antibody (294203) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human MIA
  Gly25-Gln131
  Accession # Q16674
• Specificity: Detects human MIA in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-5 or recombinant rat IL-5 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: MIA
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MIA Antibody (294203) [Unconjugated]

• CD-RAP
• Melanoma inhibitory activity protein
• melanoma inhibitory activity
• melanoma-derived growth regulatory protein
• MIA

Background

MIA, also named Cartilage-Derived Retinoic Acid-Sensitive Protein (CD-RAP), is a secreted protein that plays an important role in melanoma metastasis. It is highly expressed in malignant melanomas and is associated with tumor progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MIA Antibody (MAB20501)(3)

Publications using MAB20501

• Storkhead box 2 and melanoma inhibitory activity promote oral squamous cell carcinoma progression Oncotarget, 2016-05-03;7(18):26751-64. 2016-05-03 [PMID: 27050375] (ICC, Western Blot, Human)
• Burns JC, Kelly MC, Hoa M et al. Single-cell RNA-Seq resolves cellular complexity in sensory organs from the neonatal inner ear. Nat Commun 10/15/2015 [PMID: 26469390]
• Kolanczyk M, Mautner V, Kossler N, Nguyen R, Kuhnisch J, Zemojtel T, Jamsheer A, Wegener E, Thurisch B, Tinschert S, Holtkamp N, Park SJ, Birch P, Kendler D, Harder A, Mundlos S, Kluwe L MIA is a potential biomarker for tumour load in neurofibromatosis type 1. BMC Med, 2011-07-04;9(0):82. 2011-07-04 [PMID: 21726432] (IHC-P, Human)

Bioinformatics

• Gene Symbol: MIA
• Uniprot:
  • Q16674()